Anti-Carbonic Anhydrase III Antibody, Mouse Monoclonal

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Anti-Carbonic Anhydrase III Antibody, Mouse Monoclonal 产品信息

产品名称
Anti-Carbonic Anhydrase III Antibody, Mouse Monoclonal
经验证的应用
ELISA
交叉反应
Reacts with: Human
特异性
Human Carbonic Anhydrase III
No cross-reactivity in ELISA with
Human Carbonic Anhydrase II / CA2
Human Carbonic Anhydrase VA / CA5a
Human Carbonic Anhydrase VIII / CA8
Human Carbonic Anhydrase IX / CA9
Human Carbonic Anhydrase XIII / CA13
Human Carbonic Anhydrase XII / CA12
E.coli cell lysate
免疫原
Recombinant Human Carbonic Anhydrase III / CA3 Protein (Catalog#10503-H08E)
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Carbonic Anhydrase III / CA3 (rh Carbonic Anhydrase III / CA3; Catalog#10503-H08E; Met 1-Lys 260; NP_005172.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
来源
Monoclonal Mouse IgG1 Clone #5G8B6
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Carbonic Anhydrase III Antibody, Mouse Monoclonal 经验证的应用

应用 推荐稀释比/用量
ELISA 1:1000-1:2000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Carbonic Anhydrase III Antibody, Mouse Monoclonal: 别称

Anti-CAIII Antibody; Anti-Car3 Antibody

Carbonic Anhydrase III 背景信息

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Carbonic anhydrases (CAs) form a family of enzymes that catalyze the rapid conversion of carbon dioxide and water to bicarbonate and protons, a reaction that occurs rather slowly in the absence of a catalyst. The active site of most carbonic anhydrases contains a zinc ion, they are therefore classified as metalloenzymes. Several forms of carbonic anhydrase occur in nature. The primary function of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. Plants contain a different form called β-carbonic anhydrase, which, from an evolutionary standpoint, is a distinct enzyme, but participates in the same reaction and also uses a zinc ion in its active site. Carbonic anhydrase 3, also known as Carbonate dehydratase III, CA-III and CA3, is a cytoplasm protein which belongs to thealpha-carbonic anhydrase family. CA3 is activated by proton donors such as imidazole and the dipeptide histidylhistidine. It is inhibited by coumarins and sulfonamide derivatives such as acetazolamide. At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord.
全称
carbonic anhydrase III
参考文献
  • Ivanov, S.V. et al., 1998, Proc. Natl. Acad. Sci. USA 95:12596-601.
  • Sowden J. et al., 1998, Gene 214:157- 65.
  • Strausberg, R.L. et al., 2002, Proc. Natl. Acad. Sci. USA 99:16899-903.
  • Liao, S.Y. et al., 2003, J. Med. Genet. 40: 257-62.
  • Bataller,L. et al., 2004, Ann Neurol  56 (4): 575-9.
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