Anti-CD147 Antibody, Mouse Monoclonal

RRID Number: AB_2860104
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Anti-CD147 Antibody, Mouse Monoclonal 产品信息

产品名称
Anti-CD147 Antibody, Mouse Monoclonal
经验证的应用
ELISA,ELISA(Cap),IHC-P
交叉反应
Reacts with: Human
特异性
Human CD147
免疫原
Recombinant Human CD147 protein (Catalog#10186-H08H)
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD147. (rh CD147; Catalog#10186-H08H; NP_940991.1; Met 1-His 205). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
来源
Monoclonal Mouse IgG2a Clone #6H11C1
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CD147 Antibody, Mouse Monoclonal 经验证的应用

应用 推荐稀释比/用量
ELISA 1:1000-1:2000
IHC-P 1:50-1:200
ELISA(Cap) 1:250-1:2000
Notes
ELISA(Cap): This antibody will detect Human CD147 / EMMPRIN / Basigin in ELISA pair set (Catalog: # SEK10186). In a sandwich ELISA, it can be used as capture antibody when paired with (Catalog: # 10186-R118).
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CD147 Antibody, Mouse Monoclonal 图片

Immunochemical staining of human CD147 in human stomach with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections). The image showing membrane staining of epithelium cell.
Immunochemical staining of human CD147 in human gastric cancer with mouse monoclonal antibody (1:100, formalin-fixed paraffin embedded sections). The image showing membrane staining of epithelium cell.
Immunochemical staining of human CD147 in human esophagus with mouse monoclonal antibody (1:100, formalin-fixed paraffin embedded sections). The image showing membrane staining of squamous epithelium cell.

Anti-CD147 Antibody, Mouse Monoclonal: 别称

Anti-5F7 Antibody; Anti-Basigin Antibody; Anti-CD147 Antibody; Anti-EMMPRIN Antibody; Anti-M6 Antibody; Anti-OK Antibody; Anti-TCSF Antibody

CD147 背景信息

CD147/EMMPRIN (Extracellular Matrix Metalloproteinase Inducer), also known as Basigin (BSG), is a transmembrane glycoprotein with different forms resulted from different modes of glycosylation and N-terminal sequence variants. It is a member of the immunoglobulin superfamily with homology to both the immunoglobulin V domain and MHC class II antigen beta-chain. This protein play important roles in variety of events including spermatogenesis, embryo implantation, neural network formation. CD147 induces the production and release of matrix metalloproteinases (MMP) in the surrounding mesenchymal cells and tumor cells, and thereby promotes invasion, metastasis, growth and survival of malignant cells. Furthermore, CD147 also serves as a receptor for extracellular cyclophilinthe and its association with integrins might be important in signal transduction. Recently, CD147 displays increased expression in many cancers, and it has been previously demonstrated to participate in cancer metastasis and progression. Thus, CD147 and its antibody are used as an effective treatment for malignant cancers.
全称
basigin (Ok blood group)
参考文献
  • Tang Y, et al. (2004) Tumor-stroma interaction: positive feedback regulation of extracellular matrix metalloproteinase inducer (EMMPRIN) expression and matrix metalloproteinase-dependent generation of soluble EMMPRIN. Mol Cancer Res. 2(2): 73-80.
  • Wilson MC, et al. (2005) Basigin (CD147) is the target for organomercurial inhibition of monocarboxylate transporter isoforms 1 and 4: the ancillary protein for the insensitive MCT2 is EMBIGIN (gp70). J Biol Chem. 280(29): 27213-21.
  • Curtin KD, et al. (2005) Basigin (EMMPRIN/CD147) interacts with integrin to affect cellular architecture. J Cell Sci. 118(Pt 12): 2649-60.
  • Zhu H, et al. (2009) A novel antibody fragment targeting HAb18G/CD147 with cytotoxicity and decreased immunogenicity. Cancer Biol Ther. 8(11): 1035-44. Zhu H, et al. (2009) A novel antibody fragment targeting HAb18G/CD147 with cytotoxicity and decreased immunogenicity. Cancer Biol Ther. 8(11): 1035-44.
  • Seizer P, et al. (2009) EMMPRIN (CD147) is a novel receptor for platelet GPVI and mediates platelet rolling via GPVI-EMMPRIN interaction. Thromb Haemost. 101(4): 682-6.
  • Moonsom S, et al. (2010) A Competitive ELISA for Quantifying Serum CD147: Reduction of Soluble CD147 Levels in Cancer Patient Sera. Hybridoma (Larchmt). 29(1): 45-52.
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