Anti-CD44 Antibody, Mouse Monoclonal

RRID Number: AB_2860417

Anti-CD44 Antibody, Mouse Monoclonal 产品信息

产品名称
Anti-CD44 Antibody, Mouse Monoclonal
经验证的应用
WB,ELISA,ELISA(Cap),IHC-P,IP
交叉反应
Reacts with: Human
特异性
Human CD44
No cross-reactivity in ELISA with
Human DMP1
Human cell lysate (293 cell line)
免疫原
Recombinant Human CD44 protein (Catalog#12211-H08H)
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD44 (rh CD44; Catalog#12211-H08H; P16070-1; Met 1-Pro 220). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
来源
Monoclonal Mouse IgG1 Clone #1C11H1
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CD44 Antibody, Mouse Monoclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:1000
ELISA 1:5000-1:10000
IHC-P 1:100-1:500
IP IP:0.5-2 μL/mg of lysate
ELISA(Cap) 1:1000-1:10000
Notes
ELISA(Cap): This antibody will detect Human CD44 in ELISA pair set (Catalog: # SEK12211). In a sandwich ELISA, it can be used as capture antibody when paired with (Catalog: # 12211-MM10).
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CD44 Antibody, Mouse Monoclonal 图片

Immunochemical staining of human CD44 in human skin with mouse monoclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections. The image showing membrane staining of squamous epithelium cell.
Immunochemical staining of human CD44 in human esophagus with mouse monoclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections. The image showing membrane staining of squamous epithelium cell.
Immunochemical staining of human CD44 in human bladder carcinoma with mouse monoclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections. The image showing membrane staining of epithelium cell.

Anti-CD44 mouse monoclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:82 kDa

Observed band size:98 kDa

CD44 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

1µL anti-CD44 mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-CD44 mouse monoclonal antibody,at 1:500 dilution

Secondary antibody:

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size: 82 kDa

Observed band size :95 kDa

Anti-CD44 Antibody, Mouse Monoclonal: 别称

Anti-CDW44 Antibody; Anti-CSPG8 Antibody; Anti-ECMR-III Antibody; Anti-HCELL Antibody; Anti-HUTCH-I Antibody; Anti-IN Antibody; Anti-LHR Antibody; Anti-MC56 Antibody; Anti-MDU2 Antibody; Anti-MDU3 Antibody; Anti-MIC4 Antibody; Anti-Pgp1 Antibody

CD44 背景信息

CD44 is a type I transmembrane protein and a member of the cartilage link protein family. It is involved in cell-cell and cell-matrix interactions and signal transduction. Several CD44 ligands have been identified. The most extensively characterized ligand for CD44 is hyaluronan, a component of the extracellular matrix. CD44 protein is expressed on the majority of immune cells. The binding of CD44 to hyaluronan is induced on T lymphocytes after activation by antigen and on monocytes after stimulation by inflammatory agents. Under inflammatory conditions, CD44 on endothelial cells presents hyaluronan to CD44 on activated T lymphocytes and mediates a rolling interaction under flow conditions. Perturbations of the hyaluronan-CD44 interaction at the plasma membrane by various antagonists result in attenuation of receptor tyrosine kinase and transporter activities and inhibition of tumor progression in vivo. CD44 is known to interact with the ezrin family (ERM family) members and form a complex that plays diverse roles within both normal and abnormal cells, particularly cancer cells. CD44 and ezrin and their respective complex have properties suggesting that they may be important in the process of tumour-endothelium interactions, cell migrations, cell adhesion, tumour progression and metastasis.
全称
CD44 molecule (Indian blood group)
参考文献
  • Bajorath J. (2000) Molecular organization, structural features, and ligand binding characteristics of CD44, a highly variable cell surface glycoprotein with multiple functions. Proteins. 39(2): 103-11.
  • Johnson P, et al. (2000) A role for the cell adhesion molecule CD44 and sulfation in leukocyte-endothelial cell adhesion during an inflammatory response? Biochem Pharmacol. 59(5): 455-65.
  • Martin TA, et al. (2003) The role of the CD44/ezrin complex in cancer metastasis. Crit Rev Oncol Hematol. 46(2): 165-86.
  • Toole BP, et al. (2008) Hyaluronan, CD44 and Emmprin: partners in cancer cell chemoresistance. Drug Resist Updat. 11(3): 110-21.
  • Johnson P, et al. (2009) CD44 and its role in inflammation and inflammatory diseases. Inflamm Allergy Drug Targets. 8(3): 208-20.
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