Anti-CD46 Antibody, Mouse Monoclonal

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Anti-CD46 Antibody, Mouse Monoclonal 产品信息

产品名称
Anti-CD46 Antibody, Mouse Monoclonal
经验证的应用
FCM,ICC/IF
交叉反应
Reacts with: Human
特异性
Human CD46
免疫原
Recombinant Human CD46 protein (Catalog#12239-H08H)
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD46 (rh CD46; Catalog#12239-H08H; EAW93471.1; Met1-Asp328). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
来源
Monoclonal Mouse IgG1 Clone #05
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CD46 Antibody, Mouse Monoclonal 经验证的应用

应用 推荐稀释比/用量
ICC-IF 1:20-1:100
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CD46 Antibody, Mouse Monoclonal 图片

Profile of anti-CD46 reactivity on HeLa cells analyzed by flow cytometry.
Immunofluorescence staining of Human CD46 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human CD46 monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-mouse IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.

Anti-CD46 Antibody, Mouse Monoclonal: 别称

Anti-AHUS2 Antibody; Anti-MCP Antibody; Anti-MIC10 Antibody; Anti-TLX Antibody; Anti-TRA2.10 Antibody

CD46 背景信息

CD46, also known as Membrane Cofactor Protein (MCP), is a complement regulatory protein. CD46 is a type 1 membrane protein that plays an important inhibitory role in the complement system. CD46 is expressed in white blood cells, platelets, epithelial cells, and fibroblasts. Human CD46 shares 5% amino acid sequence identity with mouse and rat CD46. The importance of CD46 to complement regulation is underscored by the observation that genetic loss of CD46 leads to development of atypical hemolytic-uremic syndrome (aHUS), a disease characterized by uncontrolled complement activation. CD46 is implicated in the development and/or progression of selected cancer types.
全称
CD46 molecule, complement regulatory protein
Related Pathways
  • Complement Activation Pathways
    Complement Activation Pathways
参考文献
  • Lublin D.M., et al.,(1988), Molecular cloning and chromosomal localization of human membrane cofactor protein (MCP). Evidence for inclusion in the multigene family of complement-regulatory proteins. J. Exp. Med. 168:181-194.
  • Purcell D.F., et al., (1991), Alternatively spliced RNAs encode several isoforms of CD46 (MCP), a regulator of complement activation.Immunogenetics 33:335-344.
  • Post T.W., et al.,(1991), Membrane cofactor protein of the complement system: alternative splicing of serine/threonine/proline-rich exons and cytoplasmic tails produces multiple isoforms that correlate with protein phenotype.J. Exp. Med. 174:93-102.
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