Anti-CHL1 Antibody, Mouse Monoclonal

RRID Number: AB_2860094
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Anti-CHL1 Antibody, Mouse Monoclonal 产品信息

产品名称
Anti-CHL1 Antibody, Mouse Monoclonal
经验证的应用
ELISA,ELISA(Det)
交叉反应
Reacts with: Human
特异性
Human CHL1
免疫原
Recombinant Human CHL-1 Protein (Catalog#10143-H08H)
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CHL-1 (rhCHL-1; Catalog#10143-H08H; AAI04919.1; Met 1-Gln 1080). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
来源
Monoclonal Mouse IgG1 Clone #6E6E4
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CHL1 Antibody, Mouse Monoclonal 经验证的应用

应用 推荐稀释比/用量
ELISA 1:500-1:1000
ELISA(Det) 1:1000-1:10000
Notes
ELISA(Det): This antibody will detect Human CHL1 / LICAM2 / CALL in ELISA pair set (Catalog: # SEK10143). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 10143-MM05).
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CHL1 Antibody, Mouse Monoclonal: 别称

Anti-CALL Antibody; Anti-L1CAM2 Antibody; Anti-LICAM2 Antibody

CHL1 背景信息

Neural cell adhesion molecule L1-like protein, also known as close homolog of L1 (CHL1) is the prototypic member of the CTF / NF-1 family of transcription factors that serve as a novel calcium signaling pathway-responsive transcription factor and is considered as a member of the largest ctf complementation group, consisting of 30 of 126 ctf mutants isolated. CHL1 is a cell adhesion molecule highly related to L1. It contains structure plan of six extracellular C2-type immunoglobulin (Ig) domains followed by five fibronectin type Ⅲ domains linked by a single membrane-spanning region to a short cytoplasmic domain. The extracellular portion of CHL1 is higyly glycosylated and involved in hemophilic disease.
全称
cell adhesion molecule L1-like
参考文献
  • Alevizopoulos A, et al. (1997) Regulation of the Transforming Growth Factor beta-responsive Transcription Factor CTF-1 by Calcineurin and Calcium/ Calmodulin-dependent Protein Kinase IV. The Journal of Biological Chemistry. 272: 23597-605.
  • Gerring SL, et al. (1990) The CHL1 (CTF 1) gene product of Saccharomyces cerevisiae is important for chromosome transmission and normal cell cycle progression in G2 / M. EMBO J. 9 (13): 4347-58.
  • Wei MH, et al. (1998) In silico-initiated cloning and molecular characterization of a novel human member of the L1 gene family of neural cell adhesion molecules. Human Genetics. 103 (3): 355-64.
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