Anti-CRTAM Antibody, Mouse Monoclonal

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Anti-CRTAM Antibody, Mouse Monoclonal 产品信息

产品名称
Anti-CRTAM Antibody, Mouse Monoclonal
经验证的应用
FCM
交叉反应
Reacts with: Human
特异性
Human CRTAM
免疫原
Recombinant Human CRTAM Protein (Catalog#11975-H08H)
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CRTAM (rh CRTAM; Catalog#11975-H08H; NP_062550.2; Met1-Ser286). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
来源
Monoclonal Mouse IgG1 Clone #12
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CRTAM Antibody, Mouse Monoclonal 经验证的应用

应用 推荐稀释比/用量
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CRTAM Antibody, Mouse Monoclonal 图片

Flow cytometric analysis of Human CRTAM(CD355) expression on human peripheral blood lymphocytes. Human peripheral blood mononulcear cells were stimulated for 24 hours with PMA and Ionomycin, and stained with Purified anti-Human CRTAM(CD355), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphocytes.

Anti-CRTAM Antibody, Mouse Monoclonal: 别称

Anti-CD355 Antibody

CRTAM 背景信息

Cytotoxic and regulatory T-cell molecule, also known as Class-I MHC-restricted T-cell-associated molecule and CRTAM, is a single-pass type I membrane protein which belongs to thenectin family.CRTAM contains oneIg-like C2-type (immunoglobulin-like) domain and oneIg-like V-type (immunoglobulin-like) domain. In the immune system, the expression of CRTAM is restricted to activated class-I MHC-restricted cells, including NKT and CD8 cells. It is strongly expressed in spleen, thymus, small intestine, peripheral blood leukocyte, and in purkinje neurons in cerebellum. It is expressed at much lower levels in testis, ovary, colon, lung and lymphoid tissues. CRTAM is a member of the immunoglobulin superfamily that complies with the structural characteristics of the JAM family of proteins and is phylogenetically more closely related to nectin-like proteins. It is a molecule involved in epithelial cell adhesion. CRTAM is sensitive to intermediate filament disruption and treatment of monolayers with soluble CRTAM enhances cell-cell dissociation and lowers transepithelial electrical resistance. CRTAM may also induce retention by binding to CD8+ dendritic cells (DCs) at the late stage of activation before proliferation.
全称
cytotoxic and regulatory T cell molecule
参考文献
  • Garay, E. et al., 2010, J Cell Biochem. 111 (1):111-22.
  • Medina-C.O. et al., 2010, Dev Comp Immunol. 34 (2):196-202.
  • Takeuchi, A. et al., 2009, J Immunol.183 (7):4220-8.
  • Valle-Rios,R. et al., 2009, Mol Immunol. 46 (16): 3379-87.
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