PDIA4 was immunoprecipitated using:
Lane A:0.5 mg Hela Whole Cell Lysate
Lane B:0.5 mg MCF-7 Whole Cell Lysate2 µL anti-PDIA4 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein G.Primary antibody:
Anti-PDIA4 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 73 kDa
Observed band size: 73 kDa
Anti-KRT5 rabbit polyclonal antibody at 1:500 dilution
Lane A: MCF7 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:62 kDa
Observed band size:55 kDa
(We are unsure as to the identity of these extra bands.)