A synthetic peptide corresponding to the N-terminus of the Human DNMT1
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human DNMT1, and purified by antigen affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Anti-DNMT1 Antibody, Rabbit Polyclonal 经验证的应用
Please Note: Optimal concentrations/dilutions should be determined by the end user.
Anti-DNMT1 Antibody, Rabbit Polyclonal 图片
Immunofluorescence staining of DNMT1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human DNMT1 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.
Immunochemical staining of human DNMT1 in human placenta with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).
Immunochemical staining of human DNMT1 in human brain with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).
DNA methylation is mediated by a conserved family of DNA methyltransferases (Dnmts). The human genome encodes three active Dnmts (Dnmt1, Dnmt3a and Dnmt3b), the tRNA methyltransferase Dnmt2, and the regulatory protein Dnmt3L. in addition to its DNA methylating activity, DNMT1 can act on gene transcription by modulating permissive H3K4 and repressive H3K27 trimethylation in developing inhibitory interneurons similar to what was found in other cell types. In particular, the transcriptional control of Pak6 was accomplished by interactions of DNMT1 with the Polycomb-repressor complex 2 (PCR2) core enzyme EZH2, mediating repressive H3K27 trimethylation at regulatory regions of the Pak6 gene locus. DNMT1 in promoting interneuron migration through the transcriptional control over key genes like Pak6 achieved by a crosstalk with histone tail methylation.