Anti-EphB4 Antibody (FITC), Mouse Monoclonal

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Anti-EphB4 Antibody (FITC), Mouse Monoclonal 产品信息

产品名称
Anti-EphB4 Antibody (FITC), Mouse Monoclonal
经验证的应用
FCM,ICC/IF
交叉反应
Reacts with: Human
特异性
Human EphB4
免疫原
Recombinant Human EphB4 protein (Catalog#10235-H08H)
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EphB4 (rhEphB4; Catalog#10235-H08H; NP_004435.3; Met 1-Ala 539) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
来源
Monoclonal Mouse IgG1 Clone #04
纯化
Protein A
缓冲液
Aqueous solution containing 0.5% BSA and 0.09% sodium azide
偶联物
FITC
浓度
10 μl/Test, 0.1 mg/ml
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Anti-EphB4 Antibody (FITC), Mouse Monoclonal 经验证的应用

应用 推荐稀释比/用量
ICC-IF 5-20 μg/mL
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-EphB4 Antibody (FITC), Mouse Monoclonal 图片

Profile of anti-EphB4 reactivity on MCF-7 cells analyzed by flow cytometry.
Immunofluorescence staining of Human EphB4 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human EphB4 monoclonal antibody (FITC-conjugated, 10 µg/ml) at 37℃ 1 hour. Positive staining was localized to plasma membrane.

Anti-EphB4 Antibody (FITC), Mouse Monoclonal: 别称

Anti-HTK Antibody; Anti-MYK1 Antibody; Anti-TYRO11 Antibody

EphB4 背景信息

Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore, EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Therefore, Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers.
全称
EPH receptor B4
Related Pathways
  • Actin Dynamics Signaling Pathway
    Actin Dynamics Signaling Pathway
  • G Protein-coupled Receptors Signaling
    G Protein-coupled Receptors Signaling
  • MAPK-Erk Pathway
    MAPK-Erk Pathway
  • Autophagy Pathway
    Autophagy Pathway
参考文献
  • Davalos V, et al. (2006) EPHB4 and survival of colorectal cancer patients. Cancer Res. 66(18): 8943-8.
  • Zhao C, et al. (2006) Bidirectional ephrinB2-EphB4 signaling controls bone homeostasis. Cell Metab. 4(2): 111-21.
  • Kertesz N, et al. (2006) The soluble extracellular domain of EphB4 (sEphB4) antagonizes EphB4-EphrinB2 interaction, modulates angiogenesis, and inhibits tumor growth. Blood. 107(6): 2330-8.
  • Noren NK, et al. (2007) Paradoxes of the EphB4 receptor in cancer. Cancer Res. 67(9): 3994-7.
  • Taylor AC, et al. (2007) EphB4 expression along adult rat microvascular networks: EphB4 is more than a venous specific marker. Microcirculation. 14(3): 253-67.
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