Reacts with: Human Predicted to React with:
Mouse, Rat (Species predicted to react based on 100% sequence homology)
A synthetic peptide corresponding to the N-terminus of the Human ETS1
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human ETS1, and purified by antigen affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Anti-ETS1 Antibody, Rabbit Polyclonal 经验证的应用
Please Note: Optimal concentrations/dilutions should be determined by the end user.
Anti-ETS1 Antibody, Rabbit Polyclonal 图片
Immunofluorescence staining of ETS1 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human ETS1 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.
The ETS transcription factors regulate expression of genes involved in normal cell development, proliferation, differentiation, angiogenesis, and apoptosis, consisting of 28 family members in humans. Dysregulation of these transcription factors facilitates cell proliferation in cancers, and several members participate in invasion and metastasis by activating gene transcription. ETS1 and ETS2 are the founding members of the ETS family and regulate transcription by binding to ETS sequences. They are both involved in oncogenesis and tumor suppression depending on the biological situations used. ETS1 could be used as a novel marker of poor response to DDP and a potential therapeutic target for TNBC chemotherapy. the ETS1 transcription factor was found to enhance the VEGFA/VEGFR2 expression by directly binding to their promoters and facilitated FGF9-dependent elevation of VEGF-signaling which augmented the metastatic potential of Ovarian cancer (OC) cells.