Recombinant Anti-Galectin 3/LGALS3 Antibody, Rabbit Monoclonal

EliteRmab®, manufactured by recombinant technology
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Recombinant Anti-Galectin 3/LGALS3 Antibody, Rabbit Monoclonal 产品信息

产品名称
Recombinant Anti-Galectin 3/LGALS3 Antibody, Rabbit Monoclonal
经验证的应用
WB,IP
交叉反应
Reacts with: Human
特异性
Human Galectin 3/LGALS3
免疫原
Recombinant Human Galectin-3 / LGALS3 protein (Catalog#10289-HNAE)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Human Galectin-3 / LGALS3 (rh Galectin-3 / LGALS3; Catalog#10289-HNAE; P17931; Met1-Ile250).
来源
Monoclonal Rabbit IgG Clone #078
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-Galectin 3/LGALS3 Antibody, Rabbit Monoclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-Galectin 3/LGALS3 Antibody, Rabbit Monoclonal 图片

Anti-Galectin3 rabbit monoclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lane B: SW480 Whole Cell Lysate

Lane C: A431 Whole Cell Lysate

Lane D: MCF7 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:26 kDa

Observed band size:30 kDa

Galectin3 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg MCF-7 Whole Cell Lysate

Lane C:0.5 mg A431 Whole Cell Lysate

2 µL anti-Galectin3 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Galectin3 rabbit monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 30 kDa

Observed band size: 30 kDa

Recombinant Anti-Galectin 3/LGALS3 Antibody, Rabbit Monoclonal: 别称

Anti-CBP35 Antibody; Anti-GAL3 Antibody; Anti-GALBP Antibody; Anti-GALIG Antibody; Anti-L31 Antibody; Anti-LGALS2 Antibody; Anti-MAC2 Antibody

Galectin 3/LGALS3 背景信息

LGALS3 (Galectin 3) is a Protein Coding gene. This gene encodes a member of the galectin family of carbohydrate-binding proteins. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. LGALS3 is a beta-galactoside-binding lectin and plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion, and T-cell regulation. LGALS3 has an important role in tumor progression through inhibition of apoptosis. LGALS3 expression is associated with neoplastic transformation and with differentiation of monocytes to macrophages. Elevated expression of LGALS3 has been demonstrated in the synovium of rheumatoid arthritis (RA). Diseases associated with LGALS3 include Follicular Adenoma and Papillary Carcinoma.
全称
lectin, galactoside-binding, soluble, 3
参考文献
  • Dumic J, et al. (2006) Galectin-3: an open-ended story. Biochim Biophys Acta. 1760(4): 616-35.
  • Sharma UC, et al. (2004) Galectin-3 marks activated macrophages in failure-prone hypertrophied hearts and contributes to cardiac dysfunction. Circulation. 110(19): 3121-8.
  • Yan YP, et al. (2009) Galectin-3 mediates post-ischemic tissue remodeling. Brain Res. 1288: 116-24.
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