Anti-Galectin 3/LGALS3 Antibody, Rabbit Polyclonal


Anti-Galectin 3/LGALS3 Antibody, Rabbit Polyclonal 产品信息

Anti-Galectin 3/LGALS3 Antibody, Rabbit Polyclonal
Reacts with: Human
Human Galectin 3/LGALS3
Recombinant Human LGALS3 / Galectin-3 protein (Catalog#10289-HNAE)
Produced in rabbits immunized with purified, recombinant Human LGALS3 / Galectin-3 (rh LGALS3 / Galectin-3; Catalog#10289-HNAE; P17931; Met1-Ile250). LGALS3 / Galectin-3 specific IgG was purified by Human LGALS3 / Galectin-3 affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Galectin 3/LGALS3 Antibody, Rabbit Polyclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:1000-1:5000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Galectin 3/LGALS3 Antibody, Rabbit Polyclonal 图片

Anti-Galectin3 rabbit polyclonal antibody at 1:1000 dilution

Lane A: MCF7 Whole Cell Lysate

Lane B: Hela Whole Cell Lysate

Lysates/proteins at 20 μg per lane.


Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:26 kDa

Observed band size:30 kDa

Galectin3 was immunoprecipitated using:

Lane A:0.5 mg MCF-7 Whole Cell Lysate

Lane B:0.5 mg Hela Whole Cell Lysate

Lane C:0.5 mg SW480 Whole Cell Lysate

Lane D:0.5 mg A431 Whole Cell Lysate

2 µL anti-Galectin3 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Galectin3 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 30 kDa

Observed band size: 30 kDa

Anti-Galectin 3/LGALS3 Antibody, Rabbit Polyclonal: 别称

Anti-CBP35 Antibody; Anti-GAL3 Antibody; Anti-GALBP Antibody; Anti-GALIG Antibody; Anti-L31 Antibody; Anti-LGALS2 Antibody; Anti-MAC2 Antibody

Galectin 3/LGALS3 背景信息

LGALS3 (Galectin 3) is a Protein Coding gene. This gene encodes a member of the galectin family of carbohydrate-binding proteins. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. LGALS3 is a beta-galactoside-binding lectin and plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion, and T-cell regulation. LGALS3 has an important role in tumor progression through inhibition of apoptosis. LGALS3 expression is associated with neoplastic transformation and with differentiation of monocytes to macrophages. Elevated expression of LGALS3 has been demonstrated in the synovium of rheumatoid arthritis (RA). Diseases associated with LGALS3 include Follicular Adenoma and Papillary Carcinoma.
lectin, galactoside-binding, soluble, 3
  • Dumic J, et al. (2006) Galectin-3: an open-ended story. Biochim Biophys Acta. 1760(4): 616-35.
  • Sharma UC, et al. (2004) Galectin-3 marks activated macrophages in failure-prone hypertrophied hearts and contributes to cardiac dysfunction. Circulation. 110(19): 3121-8.
  • Yan YP, et al. (2009) Galectin-3 mediates post-ischemic tissue remodeling. Brain Res. 1288: 116-24.
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