Anti-GBA3 Antibody, Mouse Monoclonal

1/2
Price:
Size:
Number:

Anti-GBA3 Antibody, Mouse Monoclonal 产品信息

产品名称
Anti-GBA3 Antibody, Mouse Monoclonal
经验证的应用
FCM,ICC/IF
交叉反应
Reacts with: Human
特异性
Human GBA3
免疫原
Recombinant Human GBA3 protein (Catalog#11593-H08B)
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human GBA3 (rh GBA3; Catalog#11593-H08B; NP_066024.1; Met1-Leu469). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
来源
Monoclonal Mouse IgG2a Clone #05
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-GBA3 Antibody, Mouse Monoclonal 经验证的应用

应用 推荐稀释比/用量
ICC-IF 1:20-1:100
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-GBA3 Antibody, Mouse Monoclonal 图片

Flow cytometric analysis of Human GBA3 expression on Jurkat cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human GBA3, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Confocal immunofluorescence analysis of Human GBA3 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human GBA3 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody, countstained with Alexa Fluor® 546-conjugated phallotoxins (red) and DAPI (blue). Positive staining was localized to cytoplasm.

Anti-GBA3 Antibody, Mouse Monoclonal: 别称

Anti-CBG Antibody; Anti-CBGL1 Antibody; Anti-GBA3 Antibody; Anti-GLUC Antibody; Anti-KLrP Antibody; Anti-MGC104276 Antibody; Anti-MGC126878 Antibody

GBA3 背景信息

Cytosolic beta-glucosidase, also known as Cytosolic beta-glucosidase-like protein 1, GBA3, CBG and CBGL1 is a cytoplasm protein which belongs to theglycosyl hydrolase 1 family and Klotho subfamily. GBA3 / CBGL1 is a glycosidase probably involved in the intestinal absorption and metabolism of dietary flavonoid glycosides. GBA3 / CBGL1 is present in small intestine (at protein level). GBA3 / CBGL1 is expressed in liver, small intestine, colon, spleen and kidney. GBA3 / CBGL1 is down-regulated in renal cell carcinomas and hepatocellular carcinomas. GBA3 / CBGL1 is able to hydrolyze a broad variety of glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens. GBA3 / CBGL1 possesses beta-glycosylceramidase activity and may be involved in a nonlysosomal catabolic pathway of glycosylceramide.
全称
glucosidase, beta, acid 3 (gene/pseudogene)
参考文献
  • Yahata K., et al.,(2000), Molecular cloning and expression of a novel klotho-related protein. J. Mol. Med. 78:389-394.
  • de Graaf M., et al., (2001), Cloning and characterization of human liver cytosolic beta-glycosidase.Biochem. J. 356:907-910.
  • Berrin J.-G., et al.,(2002), Functional expression of human liver cytosolic beta-glucosidase in Pichia pastoris. Insights into its role in the metabolism of dietary glucosides.Eur. J. Biochem. 269:249-258.
添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后 U.S.A.