Anti-Glutamine Synthetase/GLUL Antibody, Rabbit Polyclonal

Anti-Glutamine Synthetase/GLUL Antibody, Rabbit Polyclonal 产品信息

产品名称
Anti-Glutamine Synthetase/GLUL Antibody, Rabbit Polyclonal
经验证的应用
WB,IHC-P,IP
交叉反应
Reacts with: Human
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
特异性
Human Glutamine Synthetase/GLUL
免疫原
A synthetic peptide corresponding to the C-terminus of the Human GLUL
制备方法
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human GLUL, and purified by antigen affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Glutamine Synthetase/GLUL Antibody, Rabbit Polyclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
IHC-P 1:2500-1:10000
IP 4-6 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Glutamine Synthetase/GLUL Antibody, Rabbit Polyclonal 图片

Immunochemical staining of human GLUL in human tonsil with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).
Immunochemical staining of human GLUL in human glioma with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).
Immunochemical staining of human GLUL in human breast with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).

GLUL was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

Lane B:0.5 mg 293T Whole Cell Lysate

2 µL anti-GLUL rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-GLUL rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 42 kDa

Observed band size: 42 kDa

Anti-GLUL rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:42 kDa

Observed band size:42 kDa

Anti-Glutamine Synthetase/GLUL Antibody, Rabbit Polyclonal: 别称

Anti-GLNS Antibody; Anti-GS Antibody; Anti-PIG43 Antibody; Anti-PIG59 Antibody

Glutamine Synthetase/GLUL 背景信息

Glutamate-ammonia ligase (GLUL) belongs to the glutamine synthetase family. GLUL was heterogeneously expressed in various breast cancer cells. GLUL may represent a novel target for selectively inhibiting p38 MAPK and ERK1/ERK2 signaling pathways and the proliferation potential of breast cancer cells.
全称
glutamate-ammonia ligase
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