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HER3 / ErbB3 Neutralizing Antibody

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HER3 / ErbB3 Neutralizing Antibody (Mouse Monoclonal 抗体) 产品信息

产品名称
HER3 / ErbB3 Neutralizing Antibody
经验证的应用
Neutralization
交叉反应
Reacts with: Human
特异性
Human HER3/ERBB3
No cross-reactivity with Mouse ErbB3 (Catalog # 51003-M08H) and Rat ErbB3 (Catalog # 80111-R08H) in ELISA assay.
免疫原
Recombinant Human HER3 / ErbB3 Protein (Catalog#10201-H08H)
制备方法
This antibody was obtained from a mouse immunized with purified, recombinant Human HER3 / ErbB3 (rh HER3 / ErbB3; Catalog#10201-H08H; NP_001973.2; Met1-Thr643) and was produced using recombinant antibody technology.
来源
Monoclonal Mouse IgG1 Clone #M908
纯化
Protein A
缓冲液
0.2 μm filtered solution in Histidine and Arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH6.0
偶联物
Unconjugated
内毒素
< 3 EU/mg
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

HER3 / ErbB3 Neutralizing Antibody (Mouse Monoclonal 抗体) 经验证的应用

Notes
Block: In a functional ELISA which immobilized recombinant Human NRG1/Fc Chimera (Catalog# 11609-H01H2) at 5 μg/mL (100 µL/well) in the plate, the Mouse Anti-Human ErBB3 Monoclonal Antibody (Catalog# 10201-M908) can block the binding of 1 μg/mL of biotinylated Human ErBB3 (Catalog# 10201-H08H-B) to Human NRG1/Fc Chimera, the EC50 is 1.30 μg/mL.
Neutralization: The neutralization activity of Erbb3 neutralizing antibody is Measured by its ability to neutralize NRG1­β1/HRG1­β1­induced proliferation in the MCF­7 human breast cancer cell line.
Please Note: Optimal concentrations/dilutions should be determined by the end user.

HER3 / ErbB3 Neutralizing Antibody: 别称

Anti-c-erbB-3 Antibody; Anti-c-erbB3 Antibody; Anti-EEBB3 Antibody; Anti-ErbB-3 Antibody; Anti-erbB3-S Antibody; Anti-HER3 Antibody; Anti-LCCS2 Antibody; Anti-MDA-BF-1 Antibody; Anti-p180-ErbB3 Antibody; Anti-p45-sErbB3 Antibody; Anti-p85-sErbB3 Antibody

HER3/ERBB3 背景信息

ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.
全称
erb-b2 receptor tyrosine kinase 3
参考文献
  • Kraus M.H., et al.,(1989), Isolation and characterization of ERBB3, a third member of the ERBB/epidermal growth factor receptor family: evidence for overexpression in a subset of human mammary tumors. Proc. Natl. Acad. Sci. U.S.A. 86:9193-9197.
  • Plowman G.D., et al., (1990), Molecular cloning and expression of an additional epidermal growth factor receptor-related gene.Proc. Natl. Acad. Sci. U.S.A. 87:4905-4909.
  • Katoh M., et al.,(1993), c-erbB3 gene encodes secreted as well as transmembrane receptor tyrosine kinase.Biochem. Biophys. Res. Commun. 192:1189-1197.
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