Anti-HER3/ERBB3 Antibody, Rabbit Polyclonal

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Anti-HER3/ERBB3 Antibody, Rabbit Polyclonal 产品信息

产品名称
Anti-HER3/ERBB3 Antibody, Rabbit Polyclonal
经验证的应用
WB,ELISA,IP
交叉反应
Reacts with: Human
特异性
Human HER3/ERBB3
免疫原
Recombinant Human ErbB3 protein (Catalog#10201-H08H)
制备方法
Produced in rabbits immunized with purified, recombinant Human ErbB3 (rh ErbB3; Catalog#10201-H08H; NP_001973.2; Met 1-Thr 643). ErbB3 specific IgG was purified by human ErbB3 affinity chromatography .
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-HER3/ERBB3 Antibody, Rabbit Polyclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:1000
ELISA 1:25000-1:50000
IP 1-2 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-HER3/ERBB3 Antibody, Rabbit Polyclonal 图片

ErbB3 was immunoprecipitated using:

Lane A:0.5 mg MCF-7 Whole Cell Lysate

0.5 µL anti-ErbB3 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-ErbB3 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 148 kDa

Observed band size: 183 kDa

Anti-ERBB3 rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Membrane Lysate whole cell lysate

Lysates/proteins at 20 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:148 kDa

Observed band size:183 kDa

(We are unsure as to the identity of these extra bands.)

Citation List

Anti-HER3/ERBB3 Antibody, Rabbit Polyclonal: 别称

Anti-c-erbB-3 Antibody; Anti-c-erbB3 Antibody; Anti-EEBB3 Antibody; Anti-ErbB-3 Antibody; Anti-erbB3-S Antibody; Anti-HER3 Antibody; Anti-LCCS2 Antibody; Anti-MDA-BF-1 Antibody; Anti-p180-ErbB3 Antibody; Anti-p45-sErbB3 Antibody; Anti-p85-sErbB3 Antibody

HER3/ERBB3 背景信息

ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.
全称
erb-b2 receptor tyrosine kinase 3
参考文献
  • Kraus M.H., et al.,(1989), Isolation and characterization of ERBB3, a third member of the ERBB/epidermal growth factor receptor family: evidence for overexpression in a subset of human mammary tumors. Proc. Natl. Acad. Sci. U.S.A. 86:9193-9197.
  • Plowman G.D., et al., (1990), Molecular cloning and expression of an additional epidermal growth factor receptor-related gene.Proc. Natl. Acad. Sci. U.S.A. 87:4905-4909.
  • Katoh M., et al.,(1993), c-erbB3 gene encodes secreted as well as transmembrane receptor tyrosine kinase.Biochem. Biophys. Res. Commun. 192:1189-1197.
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