Recombinant Anti-HIF-1 alpha Antibody, Rabbit Monoclonal

RRID Number: AB_2860397
EliteRmab®, manufactured by recombinant technology
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Recombinant Anti-HIF-1 alpha Antibody, Rabbit Monoclonal 产品信息

产品名称
Recombinant Anti-HIF-1 alpha Antibody, Rabbit Monoclonal
经验证的应用
ICC/IF
交叉反应
Reacts with: Human
特异性
Human HIF-1 alpha
免疫原
Recombinant Human HIF-1 alpha / HIF1A Protein (Catalog#11977-H07E)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Human HIF-1 alpha / HIF1A (rh HIF-1 alpha / HIF1A; Catalog#11977-H07E; Q16665-1; Arg575-Asn826).
来源
Monoclonal Rabbit IgG Clone #004
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-HIF-1 alpha Antibody, Rabbit Monoclonal 经验证的应用

应用 推荐稀释比/用量
ICC-IF 1:20-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-HIF-1 alpha Antibody, Rabbit Monoclonal 图片

Immunofluorescence staining of Human HIF1A in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human HIF1A monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus and cytoplasm.

Recombinant Anti-HIF-1 alpha Antibody, Rabbit Monoclonal: 别称

Anti-bHLHe78 Antibody; Anti-HIF-1A Antibody; Anti-HIF-1alpha Antibody; Anti-HIF1 Antibody; Anti-HIF1-ALPHA Antibody; Anti-MOP1 Antibody; Anti-PASD8 Antibody

HIF-1 alpha 背景信息

HIF-1 alpha, also known as HIF1A, contains 1 basic helix-loop-helix (bHLH) domain, 1 PAC (PAS-associated C-terminal) domain, and 2 PAS (PER-ARNT-SIM) domains. It is one of the two subunits of Hypoxia-inducible factor-1 (HIF1). HIF1 is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). HIF-1 alpha is expressed in most tissues with the highest levels in the kidney and heart. It is overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. HIF-1 alpha functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, it activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1A plays an essential role in embryonic vascularization, tumor angiogenesis, and the pathophysiology of ischemic disease. HIF-1 alpha binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires the recruitment of transcriptional coactivators such as CREBPB and EP300.
全称
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
研究领域
Related Pathways
  • p53 Pathway
    p53 Pathway
参考文献
  • Zhou Q, et al. (2011) Loss of either hypoxia inducible factor 1 or 2 promotes lung cancer cell colonization. Cell Cycle. 10(13):2233-4.
  • Krishnan J, et al. (2012) Dietary obesity-associated Hif1 alpha activation in adipocytes restricts fatty acid oxidation and energy expenditure via suppression of the Sirt2-NAD+ system. Genes Dev. 26(3):259-70.
  • Novo E, et al. (2012) The biphasic nature of hypoxia-induced directional migration of activated human hepatic stellate cells. J Pathol. 226(4):588-97.
  • Dungwa JV, et al. (2011) Overexpression of carbonic anhydrase and HIF-1 in Wilms tumours. BMC Cancer. 11:390.
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