Recombinant Anti-Hsp27/HSPB1 Antibody, Rabbit Monoclonal

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Recombinant Anti-Hsp27/HSPB1 Antibody, Rabbit Monoclonal 产品信息

产品名称
Recombinant Anti-Hsp27/HSPB1 Antibody, Rabbit Monoclonal
经验证的应用
WB,ELISA,ELISA(Cap),IP
交叉反应
Reacts with: Human
特异性
Human Hsp27/HSPB1
免疫原
Recombinant Human HSP27 protein (Catalog#10351-H08E)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Human HSP27 (rh HSP27; Catalog#10351-H08E; NP_001532.1; Met 1-Lys 205) .
来源
Monoclonal Rabbit IgG Clone #017
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-Hsp27/HSPB1 Antibody, Rabbit Monoclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
IP 1-4 μL/mg of lysate
ELISA(Cap) 1:250-1:2000
Notes
ELISA(Cap): This antibody will detect Human HSPB1 / HSP 27 in ELISA pair set (Catalog: # SEK10351). In a sandwich ELISA, it can be used as capture antibody when paired with (Catalog: # 10351-MM01).
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-Hsp27/HSPB1 Antibody, Rabbit Monoclonal 图片

HSP27 was immunoprecipitated using:

Lane A:0.5 mg Caco-2 Whole Cell Lysate

2 µL anti-HSP27 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-HSP27 rabbit monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 25 kDa

Observed band size: 25 kDa

Anti-HSP27 rabbit monoclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:23 kDa

Observed band size:26 kDa

Recombinant Anti-Hsp27/HSPB1 Antibody, Rabbit Monoclonal: 别称

Anti-CMT2F Antibody; Anti-HEL-S-102 Antibody; Anti-HMN2B Antibody; Anti-HS.76067 Antibody; Anti-Hsp25 Antibody; Anti-HSP27 Antibody; Anti-HSP28 Antibody; Anti-SRP27 Antibody

Hsp27/HSPB1 背景信息

全称
heat shock 27kDa protein 1
研究领域
Related Pathways
  • VEGF Signaling Pathway
    VEGF Signaling Pathway
  • P38 MAPK Signaling Pathway
    P38 MAPK Signaling Pathway
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