Anti-ICAM-2 Antibody, Rabbit Polyclonal

1/2
Price:
Size:
Number:

Anti-ICAM-2 Antibody, Rabbit Polyclonal 产品信息

产品名称
Anti-ICAM-2 Antibody, Rabbit Polyclonal
经验证的应用
WB,ELISA,IP
交叉反应
Reacts with: Human
特异性
Human ICAM-2
免疫原
Recombinant Human ICAM-2 / CD102 protein (Catalog#10332-H08H)
制备方法
Produced in rabbits immunized with purified, recombinant Human ICAM-2 / CD102 (rh ICAM-2 / CD102; Catalog#10332-H08H; NP_000864.2; Met 1-Gln 223). ICAM-2 / CD102 specific IgG was purified by Human ICAM-2 / CD102 affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-ICAM-2 Antibody, Rabbit Polyclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:1000
ELISA 1:25000-1:50000
IP 1-2 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-ICAM-2 Antibody, Rabbit Polyclonal 图片

ICAM2 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

0.5 µL anti-ICAM2 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-ICAM2 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 31 kDa

Observed band size: 31 kDa

Anti-ICAM2 rabbit polyclonal antibody at 1:500 dilution

Lane A: HL60 Membrane Lysate

Lysates/proteins at 20 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:31 kDa

Observed band size:47 kDa

Anti-ICAM-2 Antibody, Rabbit Polyclonal: 别称

Anti-CD102 Antibody

ICAM-2 背景信息

Intercellular adhesion molecule 2 (ICAM-2, CD102), belongs to the ICAM family consisting of three members identified as ligands for integrin receptors. It is a type I transmembrane glycoprotein with two Ig-like C2-type domains and binds to the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). As the second ligand of leukocyte function-associated antigen-1, ICAM-2 functions as a costimulatory molecule for effector cells. ICAM-2 is mainly expressed on vascular endothelial and hematopoietic cells. Interactions of ICAM-2 and the integrin receptors mediate cell adhesion in a wide range of lymphocyte, monocyte, natural killer cell, and granulocyte with other cells, and play important roles in many adhesion-dependent immune and inflammation responses, such as T cell aggregation, NK-cell cytotoxicity, and migration, lymphocyte recirculation, etc. Serum levels of ICAM-2 correlated significantly with the inflammatory and course sequences of trichinosis in mice and had a similar relationship with blood eosinophilia. So, estimation of ICAM-2 serum levels may prove useful in the diagnosis of trichinosis recent infections, and in monitoring the prognosis and response to treatment.
全称
intercellular adhesion molecule 2
参考文献
  • Weber KS, et al. (2004) Sialylation of ICAM-2 on platelets impairs adhesion of leukocytes via LFA-1 and DC-SIGN. Inflammation. 28(4): 177-88.
  • Tanaka H, et al. (2004) ICAM-2 gene therapy for peritoneal dissemination of scirrhous gastric carcinoma. Clin Cancer Res. 10(14): 4885-92.
  • Younis AI, et al. (2005) Intercellular adhesion molecule-2 (ICAM-2) in experimental trichinosis. J Egypt Soc Parasitol. 35(3): 1019-26.
添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后 U.S.A.