Anti-IFITM1/CD225 Antibody, Rabbit Polyclonal

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Anti-IFITM1/CD225 Antibody, Rabbit Polyclonal 产品信息

产品名称
Anti-IFITM1/CD225 Antibody, Rabbit Polyclonal
经验证的应用
WB,IHC-P,IP
交叉反应
Reacts with: Human
特异性
Human IFITM1/CD225
免疫原
A synthetic peptide corresponding to the N-terminus of the human CD225
制备方法
Produced in rabbits immunized with A synthetic peptide corresponding to the N-terminus of the human CD225, and purified by antigen affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-IFITM1/CD225 Antibody, Rabbit Polyclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:1000
IHC-P 1:10000-1:50000
IP 0.2-1 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-IFITM1/CD225 Antibody, Rabbit Polyclonal 图片

Immunochemical staining of human IFITM1 in human tonsil with rabbit polyclonal antibody (1:25000, formalin-fixed paraffin embedded sections).
Immunochemical staining of human IFITM1 in human colon carcinoma with rabbit polyclonal antibody (1:25000, formalin-fixed paraffin embedded sections).

CD225 was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

Lane B:0.5 mg K562 Whole Cell Lysate

0.5 µL anti-CD225 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CD225 rabbit polyclonal antibody,at 1:1000 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 15 kDa

Observed band size: 15 kDa

Anti-IFITM1 rabbit polyclonal antibody at 1:500 dilution

Lane A: K562 Whole Cell Lysate

Lane B: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:14 kDa

Observed band size:15 kDa

Anti-IFITM1/CD225 Antibody, Rabbit Polyclonal: 别称

Anti-42274 Antibody; Anti-CD225 Antibody; Anti-DSPA2a Antibody; Anti-IFI17 Antibody; Anti-LEU13 Antibody

IFITM1/CD225 背景信息

全称
interferon induced transmembrane protein 1
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