Anti-IL-33 Antibody, Mouse Monoclonal

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Anti-IL-33 Antibody, Mouse Monoclonal 产品信息

产品名称
Anti-IL-33 Antibody, Mouse Monoclonal
经验证的应用
ELISA,FCM,ICC/IF
交叉反应
Reacts with: Human
特异性
Human IL-33
No cross-reactivity in ELISA with
Human cell lysate (293 cell line)
免疫原
Recombinant Human IL-33 protein (Catalog#10368-HNAE)
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL-33 (rh IL-33; Catalog#10368-HNAE; NP_254274.1; Ser 112-Thr 270). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
来源
Monoclonal Mouse IgG2b Clone #03
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-IL-33 Antibody, Mouse Monoclonal 经验证的应用

应用 推荐稀释比/用量
ELISA 1:1000-1:2000
ICC-IF 1:20-1:100
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-IL-33 Antibody, Mouse Monoclonal 图片

Immunofluorescence staining of Human IL33 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human IL33 monoclonal antibody (1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green) and counterstained with DAPI(blue). Positive staining was localized to cytoplasm.
Flow cytometric analysis of Purified anti-Human IL-33 on HUVEC cells. HUVEC cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and stained with Purified Mouse anti-IL-33 (Bold line hisgram), then stained with a FITC-conjugated second step antibody.

Anti-IL-33 Antibody, Mouse Monoclonal: 别称

Anti-C9orf26 Antibody; Anti-DVS27 Antibody; Anti-IL1F11 Antibody; Anti-NF-HEV Antibody; Anti-NFEHEV Antibody

IL-33 背景信息

Interleukin 33 (IL-33), also known as DVS27 or NF-HEV (Nuclear Factor from High Endothelial Venules), is a pro-inflammatory protein and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 protein is expressed highly and rather selectively by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyer's patches, and lymph nodes. IL-33 protein has transcriptional regulatory properties, and the researches suggested that IL-33 is a dual-function protein that might act both as a cytokine and as an intracellular nuclear factor. As a type 2 cytokines, IL-33 protein also play a pivotal role in helminthic infection and allergic disorders.
全称
interleukin 33
Related Pathways
  • IL1 signaling pathway
    IL1 signaling pathway
参考文献
  • Iikura M, et al. (2007) IL-33 can promote survival, adhesion and cytokine production in human mast cells. Lab Invest. 87(10): 971-8.
  • Lamkanfi M, et al. (2009) IL-33 raises alarm. Immunity. 31(1): 5-7.
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