Anti-IL2RG rabbit polyclonal antibody at 1:500 dilution
Lane A: HELA Whole Cell Lysate
Lane B: RAJI Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:42 kDa
Observed band size:44 kDa
IL2RG was immunoprecipitated using:
Lane A:0.5 mg Raji Whole Cell Lysate1 µL anti-IL2RG rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-IL2RG rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 41 kDa
Observed band size: 41 kDa