Recombinant Anti-IL3RA Antibody, Rabbit Monoclonal

EliteRmab®, manufactured by recombinant technology
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Recombinant Anti-IL3RA Antibody, Rabbit Monoclonal 产品信息

产品名称
Recombinant Anti-IL3RA Antibody, Rabbit Monoclonal
经验证的应用
WB,ELISA,IP
交叉反应
Reacts with: Human
特异性
Human IL3RA
No cross-reactivity in ELISA with
Human IL6R
免疫原
Recombinant Human IL3RA protein (Catalog#10518-H08H)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Human IL3RA / CD123 (rh L3RA; Catalog#10518-H08H; NP_002174.1; Met 1-Arg 305).
来源
Monoclonal Rabbit IgG Clone #017
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-IL3RA Antibody, Rabbit Monoclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:1000
ELISA 1:25000-1:50000
IP 0.2-1 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-IL3RA Antibody, Rabbit Monoclonal 图片

Anti-IL3RA rabbit monoclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:43 kDa

Observed band size:38 kDa

IL3RA was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg MCF-7 Whole Cell Lysate

0.5 µL anti-IL3RA rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-IL3RA rabbit monoclonal antibody,at 1:350 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 43 kDa

Observed band size: 38 kDa

Recombinant Anti-IL3RA Antibody, Rabbit Monoclonal: 别称

Anti-CD123 Antibody; Anti-hIL-3Ra Antibody; Anti-IL3R Antibody; Anti-IL3RAY Antibody; Anti-IL3RX Antibody; Anti-IL3RY Antibody

IL3RA 背景信息

Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein that belongs to the type I cytokine receptor family and Type 5 subfamily. The specific alpha subunit of the interleukin-3 receptor (IL-3Ralpha, CD123) is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. The WSXWS motif of IL3RA appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box one motif of IL3RA is required for JAK interaction and/or activation. IL3RA represents a unique marker for primitive leukemic stem cells. Targeting of IL3RA may be a promising strategy for the preferential ablation of AML cells. Aberrant IL3RA expression is a good marker for monitoring of minimal residual disease. IL3RA is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. Recent studies have shown that interleukin-3 receptor alpha (CD123) is highly expressed on leukemia stem cells of patients with acute myeloid leukemia, and is correlated with tumor load and poor prognosis. CD123 was highly expressed in the bone marrow of the patients with myelodysplastic syndrome (MDS), significantly correlated with the proportion of bone marrow blasts, and thus might be the marker of MDS malignant clone. IL3RA is also a useful new marker for distinguishing B-cell disorders with circulating villous lymphocytes as its expression is characteristic of typical hairy cell leukemia (HCL) with high sensitivity and specificity.
全称
interleukin 3 receptor, alpha (low affinity)
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参考文献
  • Del Giudice I, et al. (2004) The diagnostic value of CD123 in B-cell disorders with hairy or villous lymphocytes. Haematologica. 89(3): 303-8.
  • Du X, et al. (2007) New immunotoxins targeting CD123, a stem cell antigen on acute myeloid leukemia cells. J Immunother. 30(6): 607-13.
  • Yue LZ, et al. (2010) Expression of CD123 and CD114 on the bone marrow cells of patients with myelodysplastic syndrome. Chin Med J (Engl). 123(15): 2034-2037.
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