Anti-Ki67/MKI67 Antibody, Mouse Monoclonal

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Anti-Ki67/MKI67 Antibody, Mouse Monoclonal 产品信息

产品名称
Anti-Ki67/MKI67 Antibody, Mouse Monoclonal
经验证的应用
IHC-P
交叉反应
Reacts with: Human
特异性
Human Ki67/MKI67
免疫原
A synthetic peptide corresponding to the center region of the Human Ki67 / MKI67
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the center region of the Human Ki67 / MKI67. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
来源
Monoclonal Mouse IgG1 Clone #21
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Ki67/MKI67 Antibody, Mouse Monoclonal 经验证的应用

应用 推荐稀释比/用量
IHC-P 1:500-1:2000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Ki67/MKI67 Antibody, Mouse Monoclonal 图片

Immunochemical staining of human KI-67 in human breast carcinoma with mouse monoclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Immunochemical staining of human KI-67 in human lung cancer with mouse monoclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Immunochemical staining of human KI-67 in human esophageal carcinoma with mouse monoclonal antibody (1:1000, formalin-fixed paraffin embedded sections).

Anti-Ki67/MKI67 Antibody, Mouse Monoclonal: 别称

Anti-KIA Antibody; Anti-MIB- Antibody; Anti-MIB-1 Antibody; Anti-PPP1R105 Antibody

Ki67/MKI67 背景信息

MKI67 contains 1 FHA domain and plays a key role in cell proliferation. During interphase, the MKI67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. MKI67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells. MKI67 is an excellent marker to determine the growth fraction of a given cell population. The fraction of MKI67-positive tumor cells is often correlated with the clinical course of cancer. It is also associated with ribosomal RNA transcription. Inactivation of antigen MKI67 leads to inhibition of ribosomal RNA synthesis.The MKI67 protein is a nuclear and nucleolar protein, which is tightly associated with somatic cell proliferation. Antibodies raised against the human MKI67 protein paved the way for the immunohistological assessment of cell proliferation, particularly useful in numerous studies on the prognostic value of cell growth in clinical samples of human neoplasms. MKI67 protein expression is an absolute requirement for progression through the cell-division cycle. Recently, MKI67 is proved be an independent prognostic factor for disease-free survival (HR 1.5-1.72) in multivariate analyses studies using samples from randomized clinical trials with secondary central analysis of the biomarker. MKI67 was not found to be predictive for long-term follow-up after chemotherapy. Nevertheless, high KI-67 was found to be associated with immediate pathological complete response in the neoadjuvant setting, with an LOE of II-B. MKI67 could be considered as a prognostic biomarker for therapeutic decision.

全称
marker of proliferation Ki-67
参考文献
  • Rahmanzadeh R, et al. (2007) Chromophore-assisted light inactivation of pKi-67 leads to inhibition of ribosomal RNA synthesis. Cell Prolif. 40(3):422-30.
  • Bullwinkel J, et al. (2006) Ki-67 protein is associated with ribosomal RNA transcription in quiescent and proliferating cells. J Cell Physiol. 206(3):624-35.
  • Schonk DM, et al. (1989) Assignment of the gene(s) involved in the expression of the proliferation-related Ki-67 antigen to human chromosome 10. Hum Genet. 83(3):297-9.
  • Endl E, et al., 2000, Exp Cell Res. Jun 15;257(2): 231-7.
  • Luporsi E, et al., 2012, Breast Cancer Res Treat. 132(3): 895-915.
  • Scholzen T, et al., 2000, J Cell Physiol. 182(3): 311-22.
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