Recombinant Anti-L1CAM Antibody, Rabbit Monoclonal

RRID Number: AB_2860093
EliteRmab®, manufactured by recombinant technology
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Recombinant Anti-L1CAM Antibody, Rabbit Monoclonal 产品信息

产品名称
Recombinant Anti-L1CAM Antibody, Rabbit Monoclonal
经验证的应用
ELISA(Det),IHC-P,FCM,ICC/IF
交叉反应
Reacts with: Human
特异性
Human L1CAM
免疫原
Recombinant Human CD171 / N-CAML1 / L1CAM protein (Catalog#10140-H08H)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Human CD171 / N-CAML1 / L1CAM (rh CD171 / N-CAML1 / L1CAM; Catalog#10140-H08H; NP_000416.1; Met1-Glu1120).
来源
Monoclonal Rabbit IgG Clone #014
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-L1CAM Antibody, Rabbit Monoclonal 经验证的应用

应用 推荐稀释比/用量
IHC-P 1:100-1:500
ICC-IF 1:20-1:100
FCM 1:25-1:100
ELISA(Det) 1:1000-1:10000
Notes
ELISA(Det): This antibody will detect Human CD171 / L1CAM in ELISA pair set (Catalog: # SEK10140). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 10140-R001).
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-L1CAM Antibody, Rabbit Monoclonal 图片

Immunochemical staining of human CD171 in human appendix with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to myenteric nerve plexus.
Flow cytometric analysis of human CD171 expression on HeLa cells. Cells were stained with purified anti-Human CD171, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence staining of Human CD171 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CD171 monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.

Recombinant Anti-L1CAM Antibody, Rabbit Monoclonal: 别称

Anti-CAML1 Antibody; Anti-CD171 Antibody; Anti-HSAS Antibody; Anti-HSAS1 Antibody; Anti-MASA Antibody; Anti-MIC5 Antibody; Anti-N-CAM-L1 Antibody; Anti-N-CAML1 Antibody; Anti-NCAM-L1 Antibody; Anti-S10 Antibody; Anti-SPG1 Antibody

L1CAM 背景信息

L1 cell adhesion molecule (L1CAM), also designated as CD171, is a cell adhesion receptor of the immunoglobulin superfamily, known for its roles in nerve cell function. While originally believed to be present only in brain cells, in recent years L1-CAM has been detected in other tissues, and a variety of cancer cells, including some common types of human cancer. L1CAM interacts with a variety of ligands including axonin-1, CD9, neurocan, and integrins, and it has been revealed that the RGD motif in the sixth Ig domain of L1CAM is a binding site for integrins, thus important for nuclear signaling. Disruption of L1CAM function causes three X-linked neurological syndromes, i.e. hydrocephalus, MASA syndrome (mental retardation, aphasia, shuffling gait, and adducted thumbs), and spastic paraplegia syndrome. Overexpression of L1CAM in normal and cancer cells increased motility, enhanced growth rate, and promoted cell transformation and tumorigenicity. Recent work has identified L1CAM (CD171) as a novel marker for human carcinoma progression, and a candidate for anti-cancer therapy.
全称
L1 cell adhesion molecule
参考文献
  • Meier F, et al. (2006) The adhesion molecule L1 (CD171) promotes melanoma progression. Int J Cancer. 119(3): 549-55.
  • Gavert N, et al. (2008) L1-CAM in cancerous tissues. Expert Opin Biol Ther. 8(11): 1749-57.
  • Issa Y, et al. (2009) Enhanced L1CAM expression on pancreatic tumor endothelium mediates selective tumor cell transmigration. J Mol Med. 87(1): 99-112.
  • Weidle UH, et al. (2009) L1-CAM as a target for treatment of cancer with monoclonal antibodies. Anticancer Res. 29(12): 4919-31.
  • Raveh S, et al. (2009) L1 cell adhesion molecule (L1CAM) in invasive tumors. Cancer Lett. 282(2): 137-45.
  • Wolterink S, et al. (2010) Therapeutic antibodies to human L1CAM: functional characterization and application in a mouse model for ovarian carcinoma. Cancer Res. 70(6): 2504-15.
  • Manufacture of CAR-T Cells from Mobilized Cyropreserved Peripheral Blood Stem Cell Units Depends on Monocyte Depletion
    Author
    Künkele, A;Brown, C;Beebe, A;Mgebroff, S;Johnson, AJ;Taraseviciute, A;Rolczynski, LS;Chang, CA;Finney, OC;Park, JR;Jensen, MC;
    Year
    2018
    Journal
    Biol. Blood Marrow Transplant.
    Application
    FCM
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