Recombinant Anti-Leukotriene A4 Hydrolase Antibody, Rabbit Monoclonal

EliteRmab®, manufactured by recombinant technology
1/3
Price:
Size:
Number:

Recombinant Anti-Leukotriene A4 Hydrolase Antibody, Rabbit Monoclonal 产品信息

产品名称
Recombinant Anti-Leukotriene A4 Hydrolase Antibody, Rabbit Monoclonal
经验证的应用
WB,ELISA,ICC/IF,IP
交叉反应
Reacts with: Human
特异性
Human Leukotriene A4 Hydrolase
No cross-reactivity in ELISA with
Mouse LTA4H
免疫原
Recombinant Human Leukotriene A4 Hydrolase / LTA4H protein (Catalog#10276-H08B)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Human Leukotriene A4 Hydrolase / LTA4H (rh Leukotriene A4 Hydrolase / LTA4H; Catalog#10276-H08B; NP_000886.1; Met 1-Asp 611).
来源
Monoclonal Rabbit IgG Clone #201
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-Leukotriene A4 Hydrolase Antibody, Rabbit Monoclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:1000
ELISA 1:25000-1:50000
ICC-IF 1:100-1:500
IP 1-2 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-Leukotriene A4 Hydrolase Antibody, Rabbit Monoclonal 图片

Immunofluorescence staining of Human LTA4H in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human LTA4H monoclonal antibody (1:300) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Anti-LTA4H rabbit monoclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lane B: A549 Whole Cell Lysate

Lane C: 293T Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:69 kDa

Observed band size:69 kDa

LTA4H was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg 293T Whole Cell Lysate

0.5 µL anti-LTA4H rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-LTA4H rabbit monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 110 kDa

Observed band size: 110 kDa

Recombinant Anti-Leukotriene A4 Hydrolase Antibody, Rabbit Monoclonal: 别称

Anti-LAT4 Antibody

Leukotriene A4 Hydrolase 背景信息

Leukotriene A-4 hydrolase, also known as LTA-4 hydrolase, Leukotriene A (4) hydrolase, LTA4H, and LTA4, is a cytoplasm protein that belongs to the peptidase M1 family. LTA4H hydrolyzes an epoxide moiety of leukotriene A4 (LTA-4) to form leukotriene B4 (LTB-4). This enzyme also has some peptidase activity. The leukotrienes (LTs) are a class of structurally related lipid mediators involved in the development and maintenance of inflammatory and allergic reactions. In the biosynthesis of LTs, arachidonic acid was converted into the unstable intermediate epoxide LTA4, which may, in turn, be conjugated with glutathione to form the spasmogenic LTC4, or hydrolyzed into the pro-inflammatory lipid mediator LTB4 in a reaction catalyzed by Leukotriene A4 hydrolase (LTA4H). LTB4 is a classical chemoattractant of human neutrophils and triggers adherence and aggregation of leukocytes to vascular endothelium, and also modulates immune responses. As a bifunctional zinc metalloenzyme, LTA4H also exhibits an anion-dependant arginyl aminopeptidase activity of high efficiency and specificity in addition to its epoxide hydrolase activity. LTA4H is regarded as a therapeutic target for inflammation.
全称
leukotriene A4 hydrolase
参考文献
  • Mancini, JA. et al.,1995, Eur. J. Biochem. 231: 65-71.
  • Orning, L. et al., 1994, J. Biol. Chem. 269: 11269-73.
  • Rudberg, PC. et al., 2004, J. Biol. Chem. 279: 27376-82.
  • Qiu, H. et al., 2006, Proc. Natl. Acad. Sci. USA. 103: 8161-6.
添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后 U.S.A.