Recombinant Anti-LYPD3 Antibody, Rabbit Monoclonal

RRID Number: AB_2860383
EliteRmab®, manufactured by recombinant technology
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Recombinant Anti-LYPD3 Antibody, Rabbit Monoclonal 产品信息

产品名称
Recombinant Anti-LYPD3 Antibody, Rabbit Monoclonal
经验证的应用
WB,ELISA,FCM,ICC/IF
交叉反应
Reacts with: Human
特异性
Human LYPD3
免疫原
Recombinant Human C4.4A / LYPD3 protein (Catalog#11836-H08H)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Human C4.4A / LYPD3 (rh C4.4A / LYPD3; Catalog#11836-H08H; NP_055215.2; Met 1-His 286).
来源
Monoclonal Rabbit IgG Clone #213
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-LYPD3 Antibody, Rabbit Monoclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
ICC-IF 1:20-1:100
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-LYPD3 Antibody, Rabbit Monoclonal 图片

Immunofluorescence staining of Human LYPD3 in PC3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human LYPD3 monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.
Flow cytometric analysis of Human LYPD3 expression on PC-3 cells. Cells were stained with purified anti-Human LYPD3, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Anti-LYPD3 rabbit monoclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:36 kDa

Observed band size:36 kDa

Recombinant Anti-LYPD3 Antibody, Rabbit Monoclonal: 别称

Anti-C4.4A Antibody

LYPD3 背景信息

Ly6 / PLAUR domain-containing protein 3, also known as GPI-anchored metastasis-associated protein C4.4A homolog, Matrigel-induced gene C4 protein, MIG-C4, and LYPD3, is a cell membrane protein that contains two UPAR/Ly6 domains. Human LYPD3 contains two UPAR/Ly6 domains. LYPD3 is expressed in the placenta, skin, and urothelium. It is found in suprabasal keratinocytes of chronic wounds. Weak expression of LYPD3 is found in the esophagus and peripheral blood mononuclear cells. It is found in the majority of primary and metastatic transitional cell carcinomas (TCCs) and as well in breast cancer tissues, but not in adjacent normal tissues. High expression of LYPD3 is found in the tumor component of some noninvasive superficial lesions and invasive and metastatic urothelial cancers. LYPD3 is up-regulated in migrating keratinocytes during epithelisation of incisional skin wounds. LYPD3 supports cell migration. It may be involved in urothelial cell-matrix interactions. It may also be involved in tumor progression
全称
LY6/PLAUR domain containing 3
参考文献
  • Smith B.A., et al., 2001, Cancer Res. 61:1678-85.
  • Wuerfel J., et al., 2001, Gene 262:35-41.
  • Clark H.F., et al., 2003, Genome Res. 13:2265-70.
  • Fletcher G.C., et al., 2003, Br. J. Cancer 88:579-85.
  • Hansen L.V., et al., 2004, Biochem. Eng. J. 380:845-57.
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