Recombinant Anti-Marapsin Antibody, Rabbit Monoclonal

EliteRmab®, manufactured by recombinant technology
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Recombinant Anti-Marapsin Antibody, Rabbit Monoclonal 产品信息

产品名称
Recombinant Anti-Marapsin Antibody, Rabbit Monoclonal
经验证的应用
WB,IP
交叉反应
Reacts with: Human
特异性
Human Marapsin
免疫原
Recombinant Human Pancreasin / Marapsin / PRSS27 protein (Catalog#12116-H08H)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Human Pancreasin / Marapsin / PRSS27 (rh Pancreasin / Marapsin / PRSS27; Catalog#12116-H08H; Q9BQR3; Met1-Lys290).
来源
Monoclonal Rabbit IgG Clone #107
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-Marapsin Antibody, Rabbit Monoclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
IP 4-6 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-Marapsin Antibody, Rabbit Monoclonal 图片

Anti-PRSS27 rabbit monoclonal antibody at 1:500 dilution

Lane A: K562 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:32 kDa

Observed band size:32 kDa

PRSS27 was immunoprecipitated using:

Lane A:0.5 mg K562 Whole Cell Lysate

2 µL anti-PRSS27 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-PRSS27 rabbit monoclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 32 kDa

Observed band size: 32 kDa

Recombinant Anti-Marapsin Antibody, Rabbit Monoclonal: 别称

Anti-CAPH2 Antibody; Anti-MPN Antibody; Anti-UNQ1884/PRO4327 Antibody

Marapsin 背景信息

The name "Pancreasin" because it is transcribed strongly in the pancreas. This secreted, tryptic serine protease, also known as Marapsin or PRSS27 (Protease, serine, 27), is a member of the peptidase S1 family. Pancreasin is inhibited by benzamidine and leupeptin but resists several classic inhibitors of trypsin. Marapsin was constitutively expressed in nonkeratinizing stratified squamous epithelia of human esophagus, tonsil, cervix, larynx, and cornea. In fact, marapsin was the second most strongly up-regulated protease in psoriatic lesions, where expression was localized to the upper region of the hyperplastic epidermis. Similarly, in the hyperproliferative epithelium of regenerating murine skin wounds, marapsin localized to the suprabasal layers, where keratinocytes undergo squamous differentiation. Marapsin's restricted expression, localization, and cytokine-inducible expression suggest a role in the terminal differentiation of keratinocytes in hyperproliferating squamous epithelia.
全称
protease, serine 27
参考文献
  • Bhagwandin VJ, et al. (2003) Structure and activity of human pancreasin, a novel tryptic serine peptidase expressed primarily by the pancreas. J Biol Chem. 278(5): 3363-71.
  • Li W, et al. (2009) The serine protease marapsin is expressed in stratified squamous epithelia and is up-regulated in the hyperproliferative epidermis of psoriasis and regenerating wounds. J Biol Chem. 284(1): 218-28.
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