Recombinant Anti-MAX Antibody, Rabbit Monoclonal

EliteRmab®, manufactured by recombinant technology
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Recombinant Anti-MAX Antibody, Rabbit Monoclonal 产品信息

产品名称
Recombinant Anti-MAX Antibody, Rabbit Monoclonal
经验证的应用
WB,ELISA,FCM,ICC/IF,IP
交叉反应
Reacts with: Human
特异性
Human MAX
免疫原
Recombinant Human MAX / MYC associated factor X protein (Catalog#12885-H20B)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Human MAX / MYC associated factor X (rh MAX / MYC associated factor X ; Catalog#12885-H20B; NP_002373; Met1-Ser160).
来源
Monoclonal Rabbit IgG Clone #103
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-MAX Antibody, Rabbit Monoclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:1000
ELISA 1:25000-1:50000
ICC-IF 1:100-1:500
FCM 1:100-1:500
IP 0.2-1 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-MAX Antibody, Rabbit Monoclonal 图片

Immunofluorescence staining of Human MAX in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human MAX monoclonal antibody (1:300) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus.
Flow cytometric analysis of Human MAX expression on Raji cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human MAX, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

MAX was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg 293T Whole Cell Lysate

0.5 µL anti-MAX rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-MAX rabbit monoclonal antibody,at 1:1000 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 23 kDa

Observed band size: 23 kDa

Anti-MAX rabbit monoclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lane B: 293T Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:18 kDa

Observed band size:20 kDa

(We are unsure as to the identity of these extra bands.)

Recombinant Anti-MAX Antibody, Rabbit Monoclonal: 别称

Anti-bHLHd4 Antibody

MAX 背景信息

MYC associated factor X contains 1 basic helix-loop-helix (bHLH) domain and belongs to the MAX family. It is highly expressed in the brain, heart, and lung while lower levels are seen in the liver, kidney, and skeletal muscle. MYC associated factor X can form homodimers and heterodimers with other family members, which include Mad, Mxi1, and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation, and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. MYC associated factor X may also repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. Multiple alternatively spliced transcript variants have been described for MYC associated factor X gene but the full-length nature for some of them is unknown.
全称
MYC associated factor X
Related Pathways
  • P38 MAPK Signaling Pathway
    P38 MAPK Signaling Pathway
参考文献
  • Mac Partlin M, et al. (2003) Interactions of the DNA mismatch repair proteins MLH1 and MSH2 with c-MYC and MAX. Oncogene. 22(6):819-25.
  • Cheng SW, et al. (1999) c-MYC interacts with INI1/hSNF5 and requires the SWI/SNF complex for transactivation function. Nat enet. 22(1):102-5.
  • McMahon SB, et al. (1998) The novel ATM-related protein TRRAP is an essential cofactor for the c-Myc and E2F oncoproteins. Cell. 94(3):363-74.
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