Anti-NBL1 Antibody, Mouse Monoclonal

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Anti-NBL1 Antibody, Mouse Monoclonal 产品信息

产品名称
Anti-NBL1 Antibody, Mouse Monoclonal
经验证的应用
ELISA(Det),IHC-P
交叉反应
Reacts with: Human
特异性
Human NBL1
免疫原
Recombinant Human NBL1 / DAND1 / DAN protein (Catalog#10169-H08H)
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human NBL1 / DAND1 / DAN (rh NBL1 / DAND1 / DAN; Catalog#10169-H08H; NP_005371.1; Met1-Asp180). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
来源
Monoclonal Mouse IgG1 Clone #01
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-NBL1 Antibody, Mouse Monoclonal 经验证的应用

应用 推荐稀释比/用量
IHC-P 1:50-1:200
ELISA(Det) 1:1000-1:10000
Notes
ELISA(Det): This antibody will detect Human NBL1 / DAND1 in ELISA pair set (Catalog: # SEK10169). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 10169-MM06).
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-NBL1 Antibody, Mouse Monoclonal 图片

Immunochemical staining of human DAN in human lung with mouse monoclonal antibody (1:100, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
Immunochemical staining of human DAN in human liver with mouse monoclonal antibody (1:100, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
Immunochemical staining of human DAN in human kidney with mouse monoclonal antibody (1:100, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).

Anti-NBL1 Antibody, Mouse Monoclonal: 别称

Anti-D1S1733E Antibody; Anti-DAN Antibody; Anti-DAND1 Antibody; Anti-NB Antibody; Anti-NO3 Antibody

NBL1 背景信息

The Dan (Differential screening-selected gene aberrative in neuroblastoma, also known as N03) gene was first identified as the putative rat tumor suppressor gene and encodes a protein structurally related to Cerberus and Gremlin in the vertebrates. It is a founding member of the DAN family of secreted proteins, acts as an inhibitor of cell cycle progression, and is closely involved in retinoic acid-induced neuroblastoma differentiation. There are at least five mammalian protein members in the evolutionarily conserved Dan family including DAN, Gremlin/DRM, Cer1 (Cerberus-related), Dante, and PRDC (protein related to DAN and Cerberus), and share the C-terminal cystine-knot motif. As a secreted glycoprotein, DAN is a member of a class of glycoproteins shown to be secreted inhibitors of the transforming growth factor-beta (TGF-beta) and bone morphogenic protein pathways. It binds to BMPs and preventing their interactions with signaling receptor complexes, and accordingly regulates the processes of embryonic development and tissue differentiation. DAN gene product may have an important role in the regulation of the entry of cells into the S phase. Besides, the DAN gene product possesses an ability to revert phenotypes of transformed rat fibroblasts and represents a candidate tumor suppressor gene for neuroblastoma.
全称
neuroblastoma 1, DAN family BMP antagonist
参考文献
  • Ozaki T, et al. (1995) Overexpression of DAN gene product in normal rat fibroblasts causes a retardation of the entry into the S phase. Cancer Res. 55(4): 895-900.
  • Nakamura Y, et al. (1997) A product of DAN, a novel candidate tumour suppressor gene, is secreted into culture medium and suppresses DNA synthesis. Eur J Cancer. 33(12): 1986-90.
  • Ogita J, et al. (2001) Expression of the Dan gene during chicken embryonic development. Mech Dev. 109(2): 363-5.
  • Kim AS, et al. (2003) Expression of the BMP antagonist Dan during murine forebrain development. Brain Res Dev Brain Res. 145(1): 159-62.
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