Anti-NF-kB p65 Antibody, Mouse Monoclonal

RRID Number: AB_2860400
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Anti-NF-kB p65 Antibody, Mouse Monoclonal 产品信息

产品名称
Anti-NF-kB p65 Antibody, Mouse Monoclonal
经验证的应用
ELISA,FCM,ICC/IF
交叉反应
Reacts with: Human
特异性
Human NF-kB p65
免疫原
Recombinant Human RELA / Transcription factor p65 protein (Catalog#12054-H09E)
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human RELA / Transcription factor p65 (rh RELA / Transcription factor p65; Catalog#12054-H09E; Q04206-1; Met 1-Tyr 306). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
来源
Monoclonal Mouse IgG2a Clone #04
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-NF-kB p65 Antibody, Mouse Monoclonal 经验证的应用

应用 推荐稀释比/用量
ELISA 1:1000-1:2000
ICC-IF 1:20-1:100
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-NF-kB p65 Antibody, Mouse Monoclonal 图片

Immunofluorescence staining of human RELA (NF-KB p65) in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-human RELA monoclonal antibody (1:100) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Flow cytometric analysis of Purified anti-Human RelA/NFkB p65 on Jurkat cells (Left panel) and HeLa cells (Right panel).

Anti-RELA mouse monoclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lane B: Molt-4 Whole Cell Lysate

Lane C: Daudi Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:65 kDa

Observed band size:35 kDa

Anti-NF-kB p65 Antibody, Mouse Monoclonal: 别称

Anti-NFKB3 Antibody; Anti-p65 Antibody

NF-kB p65 背景信息

RELA (v-rel reticuloendotheliosis viral oncogene homolog A), also known as Nuclear factor NF-kappa-B p65 subunit, or Transcription factor p65, is a transcription factor expressed in growth plate chondrocytes where it facilitates chondrogenesis. The v-rel avian reticuloendotheliosis viral oncogene homolog A (RELA) gene encodes the major component of the NF-?B complex. NF-kappaB is a generic name for an evolutionarily conserved transcription-factor system that contributes to the mounting of an effective immune response but is also involved in the regulation of cell proliferation, development, and apoptosis. The implication of NF-kappaB in central biological processes and its extraordinary connectivity to other signaling pathways raise a need for highly controlled regulation of NF-kappaB activity at several levels. The mammalian Rel/NF-kappaB family of transcription factors, including RelA, c-Rel, RelB, NF-kappaB1 (p50 and its precursor p105), and NF-kappaB2 (p52 and its precursor p100), plays a central role in the immune system by regulating several processes ranging from the development and survival of lymphocytes and lymphoid organs to the control of immune responses and malignant transformation.
全称
v-rel avian reticuloendotheliosis viral oncogene homolog A
Related Pathways
  • Death Receptor Signaling
    Death Receptor Signaling
  • EGFR Signaling Pathway
    EGFR Signaling Pathway
  • NF-kB (NFkB) Pathway
    NF-kB (NFkB) Pathway
  • TNF Signaling
    TNF Signaling
  • B Cell Receptor Signaling Pathway
    B Cell Receptor Signaling Pathway
  • P38 MAPK Signaling Pathway
    P38 MAPK Signaling Pathway
  • T Cell Receptor Signaling Pathway
    T Cell Receptor Signaling Pathway
  • IL1 signaling pathway
    IL1 signaling pathway
  • IL17 signaling pathway
    IL17 signaling pathway
参考文献
  • Hashimoto R, et al. (2011) Variants of the RELA gene are associated with schizophrenia and their startle responses. Neuropsychopharmacology. 36(9): 1921-31.
  • Vallabhapurapu S, et al. (2009) Regulation and function of NF-kappaB transcription factors in the immune system. Annu Rev Immunol. 27: 693-733.
  • Schmitz ML, et al. (2004) NF-kappaB: a multifaceted transcription factor regulated at several levels. Chembiochem. 5(10): 1348-58.
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