Recombinant Anti-OTUB2 Antibody, Rabbit Monoclonal

EliteRmab®, manufactured by recombinant technology
1/1
Price:
Size:
Number:

Recombinant Anti-OTUB2 Antibody, Rabbit Monoclonal 产品信息

产品名称
Recombinant Anti-OTUB2 Antibody, Rabbit Monoclonal
经验证的应用
ELISA,ICC/IF
交叉反应
Reacts with: Human
特异性
Human OTUB2
免疫原
Recombinant Human OTUB2 Protein (Catalog#13177-H07E)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Human OTUB2 (rh OTUB2; Catalog#13177-H07E; Q96DC9-1; Met1-His234).
来源
Monoclonal Rabbit IgG Clone #139
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-OTUB2 Antibody, Rabbit Monoclonal 经验证的应用

应用 推荐稀释比/用量
ELISA 1:5000-1:10000
ICC-IF 1:20-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-OTUB2 Antibody, Rabbit Monoclonal 图片

Immunofluorescence staining of Human OTUB2 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human OTUB2 monoclonal antibody (1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 549-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to cytoplasm and nucleus.

Recombinant Anti-OTUB2 Antibody, Rabbit Monoclonal: 别称

Anti-C14orf137 Antibody; Anti-OTB2 Antibody; Anti-OTU2 Antibody

OTUB2 背景信息

Otubain 2 (OTUB2) is a member of DUBs that belong to the ovarian tumour (OTU) superfamily of proteins which consists of a five-stranded β-sheet sandwiched in between a small helical amino-terminal region consisting of α1 and α2, and a large helical region comprised of α3-α10. Like other DUBs, otubain 2 (OTUB2) cleaves proteins precisely at the ubiquitin-protein bond so that ubiquitylation process can be reversed and regulated. Otubain 2 (OTUB2)'s active-site cleft is sterically occluded by a novel loop conformation resulting in an oxyanion hole, which consists uniquely of backbone amides. Furthermore, the residues that orient and stabilize the active-site histidine of otubain 2 (OTUB2) are different from other cysteine proteases. This reorganization of the active-site topology provides a possible explanation for the low turnover and substrate specificity of the otubains.
全称
OTU deubiquitinase, ubiquitin aldehyde binding 2
参考文献
  • Balakirev MY, et al. (2003) Otubains: a new family of cysteine proteases in the ubiquitin pathway. EMBO Rep 4 (5): 517-22.
  • Nanao MH. (2004) Crystal structure of human otubain 2. EMBO reports. 5: 783-8.
添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后 U.S.A.