Recombinant Anti-PPM1G Antibody, Rabbit Monoclonal

EliteRmab®, manufactured by recombinant technology
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Recombinant Anti-PPM1G Antibody, Rabbit Monoclonal 产品信息

产品名称
Recombinant Anti-PPM1G Antibody, Rabbit Monoclonal
经验证的应用
WB,ELISA,IP
交叉反应
Reacts with: Human
特异性
Human PPM1G
免疫原
Recombinant Human PPM1G protein (Catalog#11245-H07E)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Human PPM1G (rh PPM1G; Catalog#11245-H07E; O15355; Met317-Asp546).
来源
Monoclonal Rabbit IgG Clone #001
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-PPM1G Antibody, Rabbit Monoclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-PPM1G Antibody, Rabbit Monoclonal 图片

Anti-PPM1G rabbit monoclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lane B: 293T Whole Cell Lysate

Lane C: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:59 kDa

Observed band size:70 kDa

PPM1G was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg 293T Whole Cell Lysate

Lane C:0.5 mg Jurkat Whole Cell Lysate

2 µL anti-PPM1G rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-PPM1G rabbit monoclonal antibody,at 1:50 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 59 kDa

Observed band size: 72 kDa

Recombinant Anti-PPM1G Antibody, Rabbit Monoclonal: 别称

Anti-PP2CG Antibody; Anti-PP2CGAMMA Antibody; Anti-PPP2CG Antibody

PPM1G 背景信息

Protein phosphatase 1G, also known as Protein phosphatase 1C, Protein phosphatase 2C isoform gamma, Protein phosphatase magnesium-dependent 1 gamma, PP2C-gamma, PPM1G and PPM1C, is a cytoplasm protein that belongs to the PP2C family. PPM1G / PP2C-gamma is widely expressed. It is most abundant in testis, skeletal muscle, and heart. Alternatively spliced transcript variants encoding the same protein have been described. PP2C family members are known to be negative regulators of cell stress response pathways. PPM1G / PP2C-gamma is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. PPM1G / PP2C-gamma also plays a role in regulating cell cycle progression.
全称
protein phosphatase, Mg2+/Mn2+ dependent, 1G
参考文献
  • Travis S.M., et al., 1997, FEBS Lett. 412:415-9.
  • Molina H., et al., 2007, Proc. Natl. Acad. Sci. USA. 104: 2199-204.
  • Matsuoka S., et al., 2007, Science 316:1160-6.
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