Anti-RAD18 Antibody, Rabbit Polyclonal

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Anti-RAD18 Antibody, Rabbit Polyclonal 产品信息

产品名称
Anti-RAD18 Antibody, Rabbit Polyclonal
经验证的应用
IHC-P,ICC/IF
交叉反应
Reacts with: Human
特异性
Human RAD18
免疫原
A synthetic peptide corresponding to the C-terminus of the Human RAD18
制备方法
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human RAD18, and purified by antigen affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-RAD18 Antibody, Rabbit Polyclonal 经验证的应用

应用 推荐稀释比/用量
IHC-P 1:500-1:2000
ICC-IF 1:300-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-RAD18 Antibody, Rabbit Polyclonal 图片

Immunofluorescence staining of RAD18 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human RAD18 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.
Immunochemical staining of human RAD18 in human testis with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).

Anti-RAD18 Antibody, Rabbit Polyclonal: 别称

Anti-RNF73 Antibody

RAD18 背景信息

RAD18, a RING-type ubiquitin ligase (E3) that plays an essential role in post-replication repair, possesses distinct domains named RING, UBZ, SAP and the RAD6-binding domain (R6BD) and forms a dimer. RAD18, a DNA damage-activated E3 ubiquitin ligase, is known to play a critical role in DNA damage repair in cancer cells. The ubiquitin ligase RAD18 is involved in post replication repair pathways via its recruitment to stalled replication forks, and its role in the ubiquitylation of proliferating cell nuclear antigen (PCNA).RAD18 is an ubiquitin ligase involved in replicative damage bypass and DNA double-strand break (DSB) repair processes. The RAD18 gene, located on the human chromosome 3p24-p25, plays a crucial role in post-replication repair (PRR) in various organisms from yeast to humans. In the human RAD18 gene, one coding single nucleotide polymorphism (SNP) at codon 302, encoding either arginine (Arg, CGA) or glutamine (Gln, CAA), was reported.
全称
RAD18, E3 ubiquitin protein ligase
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