Anti-RbAp48 Antibody, Rabbit Polyclonal

Anti-RbAp48 Antibody, Rabbit Polyclonal 产品信息

产品名称
Anti-RbAp48 Antibody, Rabbit Polyclonal
经验证的应用
WB,IHC-P,ICC/IF
交叉反应
Reacts with: Human, Mouse
特异性
Human RbAp48
免疫原
A synthetic peptide corresponding to the C-terminus of the Human RBBP4 / RBAP48
制备方法
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human RBBP4 / RBAP48, and purified by antigen affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-RbAp48 Antibody, Rabbit Polyclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
IHC-P 1:1000-1:5000
ICC-IF 1:300-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-RbAp48 Antibody, Rabbit Polyclonal 图片

Immunofluorescence staining of RBBP4 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human RBBP4 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.
Immunochemical staining RBBP4 in human testis with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).
Immunochemical staining RBBP4 in human lung cancer with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).
Immunochemical staining RBBP4 in human breast carcinoma with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).

Anti-RBBP4 rabbit polyclonal antibody at 1:500 dilution.

Lane A: HepG2 Whole Cell Lysate

Lane B: K562 Whole Cell Lysate

Lane C: Mouse spleen tissue lysate

Lane D: Rat lung tissue lysate

Lysates/proteins at 30 ug per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:48 kDa

Observed band size:55 kDa

Immunochemical staining RBBP4 in mouse kidney with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining RBBP4 in mouse lung with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.

Anti-RbAp48 Antibody, Rabbit Polyclonal: 别称

Anti-lin-53 Antibody; Anti-NURF55 Antibody; Anti-RBAP48 Antibody

RbAp48 背景信息

Histone-binding protein RBBP4, also known as Retinoblastoma-binding protein 4, Retinoblastoma-binding protein p48, Chromatin assembly factor 1 subunit C, Chromatin assembly factor I p48 subunit, Nucleosome-remodeling factor subunit RBAP48 and RBBP4, is a nucleus protein which belongs to the WD repeat RBAP46/RBAP48/MSI1 family. RBBP4 is a core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. RBBP4 is a component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling and the NURF (nucleosome remodeling factor) complex. One common myth is that age-related memory loss is an early indication of Alzheimer's disease. But researchers at the Columbia University Medical Center in New York City have found a specific protein, RbAp48, that they believe is responsible for age-related memory problems. What's more, by replenishing RbAp48 in the brains of mice, the researchers were able to undo existing age-related memory damage. To find RbAp48, researchers focused on the hippocampus, the region of the brain where memories are formed. After studying eight healthy brains donated to science by people between the ages of 33 and 88, they found that RbAp48 was reduced by nearly 5 percent in the older brains. The researchers found that when they turned off RbAp48 in younger mice, they became more forgetful, while increasing RbAp48 in older mice restored memory. The mice were given memory tests that included object recognition and water maze problems.
全称
retinoblastoma binding protein 4
参考文献
  • Rasmussen HH. et al.,1992, Electrophoresis 13:960-9.
  • Qian Y.-W. et al., 1993,  Nature 364:648-52.
  • Yarden R.I. et al., 1999, Proc. Natl. Acad. Sci. USA. 96: 4983-8.
  • Ota T. et al., 2004, Nat. Genet. 36:40-45.
  • Gauci S. et al., 2009, Anal. Chem. 81:4493-501.
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