Recombinant Anti-Serum Albumin Antibody, Rabbit Monoclonal

RRID Number: AB_2860536
EliteRmab®, manufactured by recombinant technology
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Recombinant Anti-Serum Albumin Antibody, Rabbit Monoclonal 产品信息

产品名称
Recombinant Anti-Serum Albumin Antibody, Rabbit Monoclonal
经验证的应用
WB,ELISA,FCM,ICC/IF,IP
交叉反应
Reacts with: Human
特异性
Human Serum Albumin
No cross-reactivity in ELISA with
Bovine serum albumin / BSA
免疫原
Human Serum Albumin / HSA
制备方法
This antibody was obtained from a rabbit immunized with purified Human Serum Albumin.
来源
Monoclonal Rabbit IgG Clone #101
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-Serum Albumin Antibody, Rabbit Monoclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
ICC-IF 1:20-1:100
FCM 1:25-1:100
IP 0.5-2 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-Serum Albumin Antibody, Rabbit Monoclonal 图片

Immunofluorescence staining of Human HSA in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human HSA monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.
Flow cytometric analysis of Human HSA expression on HepG2 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human HSA, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

HAS was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-HAS rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-HAS rabbit monoclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 68 kDa

Observed band size: 68 kDa

Anti-HAS rabbit monoclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:68 kDa

Observed band size:68 kDa

Recombinant Anti-Serum Albumin Antibody, Rabbit Monoclonal: 别称

Anti-ANALBA Antibody; Anti-FDAH Antibody; Anti-PRO0883 Antibody; Anti-PRO0903 Antibody; Anti-PRO1341 Antibody

Serum Albumin 背景信息

全称
albumin
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