Anti-SUMO2 Antibody, Rabbit Polyclonal

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Anti-SUMO2 Antibody, Rabbit Polyclonal 产品信息

产品名称
Anti-SUMO2 Antibody, Rabbit Polyclonal
经验证的应用
WB,ICC/IF,IP
交叉反应
Reacts with: Human
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
特异性
Human SUMO2
免疫原
A synthetic peptide corresponding to the C-terminus of the Human SUMO2
制备方法
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human SUMO2, and purified by antigen affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-SUMO2 Antibody, Rabbit Polyclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
ICC-IF 1:300-1:10000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-SUMO2 Antibody, Rabbit Polyclonal 图片

Immunofluorescence staining of SUMO2 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human SUMO2 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus..

SUMO2 was immunoprecipitated using:

Lane A:0.5 mg SW480 Whole Cell Lysate

2 µL anti-SUMO2 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-SUMO2 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 17 kDa

Observed band size: 17 kDa

Anti-SUMO2 rabbit polyclonal antibody at 1:500 dilution

Lane A: SW480 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:12 kDa

Observed band size:17 kDa

(We are unsure as to the identity of these extra bands.)

Anti-SUMO2 Antibody, Rabbit Polyclonal: 别称

Anti-HSMT3 Antibody; Anti-Smt3A Antibody; Anti-SMT3B Antibody; Anti-SMT3H2 Antibody; Anti-SUMO3 Antibody

SUMO2 背景信息

SUMO2 gene silencing promotes proliferation, and inhibits the apoptosis and senescence of NPCs in rats with IDD through the down-regulation of the p53 signaling pathway. Thus, SUMO2 is a potential target in the treatment of Intervertebral disc degeneration (IDD). The small ubiquitin-related modifier protein (SUMO) functions as a posttranslational modification system and SUMO2/3 subtypes are often activated in early stress. SUMO2 as a novel activator of Cn-NFAT signaling in cardiomyocytes.
全称
small ubiquitin-like modifier 2
Related Pathways
  • NF-kB (NFkB) Pathway
    NF-kB (NFkB) Pathway
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