Anti-Syndecan-1/CD138 Antibody, Rabbit Polyclonal

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Anti-Syndecan-1/CD138 Antibody, Rabbit Polyclonal 产品信息

产品名称
Anti-Syndecan-1/CD138 Antibody, Rabbit Polyclonal
经验证的应用
WB,IHC-P
交叉反应
Reacts with: Human
特异性
Human Syndecan-1/CD138
免疫原
Recombinant Human Syndecan-1 / SDC1 / CD138 protein (Catalog#11429-H08H)
制备方法
Produced in rabbits immunized with purified, recombinant Human Syndecan-1 / SDC1 / CD138 (rh Syndecan-1 / SDC1 / CD138; Catalog#11429-H08H; NP_002988.3; Met 1-Glu 251). Syndecan-1 / SDC1 / CD138 specific IgG was purified by Human Syndecan-1 / SDC1 / CD138 affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Syndecan-1/CD138 Antibody, Rabbit Polyclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
IHC-P 1:500-1:2000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Syndecan-1/CD138 Antibody, Rabbit Polyclonal 图片

Immunochemical staining of human SDC1 in human kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Immunochemical staining of human SDC1 in human colon cancer with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to glandular epithelium.
Immunochemical staining of human SDC1 in human breast cancer with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to glandular epithelium.

Anti-SDC1 rabbit polyclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lane B: 293T Whole Cell Lysate

Lane C: MCF-7 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:32 kDa

Observed band size:55 kDa

Anti-Syndecan-1/CD138 Antibody, Rabbit Polyclonal: 别称

Anti-CD138 Antibody; Anti-SDC Antibody; Anti-SYND1 Antibody; Anti-syndecan Antibody; Anti-Syndecan-1 Antibody

Syndecan-1/CD138 背景信息

Syndecan-1 also known as SDC1 and CD138, is the most extensively studied member of the syndecan family. It is found mainly in epithelial cells, but its expression is developmentally regulated during embryonic development. Syndecan-1/SDC1/CD138 has been shown to mediate cell adhesion to several ECM molecules, and to act as a coreceptor for fibroblast growth factors, potent angiogenic growth factors involved also in differentiation. Syndecan-1/SDC1/CD138 expression is reduced during malignant transformation of various epithelia, and this loss correlates with the histological differentiation grade of squamous cell carcinomas, lacking from poorly differentiated tumours. In squamous cell carcinomas of the head and neck, positive syndecan-1 expression correlates with a more favourable prognosis. Experimental studies on the role of Syndecan-1 in malignant transformation have shown that Syndecan-1/SDC1/CD138 expression is associated with the maintenance of epithelial morphology, anchorage-dependent growth and inhibition of invasiveness in vitro.
全称
syndecan 1
参考文献
  • Inki P, et al. (1996) The role of syndecan-1 in malignancies. Ann Med. 28(1): 63-7.
  • Subramanian SV, et al. (1997) Regulated shedding of syndecan-1 and -4 ectodomains by thrombin and growth factor receptor activation. J Biol Chem. 272(23): 14713-20.
  • Park PW, et al. (2001) Exploitation of syndecan-1 shedding by Pseudomonas aeruginosa enhances virulence. Nature. 411(6833): 98-102.
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