Recombinant Anti-TFPI Antibody, Rabbit Monoclonal

EliteRmab®, manufactured by recombinant technology
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Recombinant Anti-TFPI Antibody, Rabbit Monoclonal 产品信息

产品名称
Recombinant Anti-TFPI Antibody, Rabbit Monoclonal
经验证的应用
WB,IP
交叉反应
Reacts with: Human
特异性
Human TFPI
No cross-reactivity in ELISA with
Mouse TFPI / LACI / EPI
Human TFPI2 / PP5
Human Vitronectin / VTN
免疫原
Recombinant Human TFPI Protein (Catalog#10564-H08H)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Human TFPI / LACI (rh TFPI; Catalog#10564-H08H; NP_006278.1; Met 1-Lys 282).
来源
Monoclonal Rabbit IgG Clone #6
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-TFPI Antibody, Rabbit Monoclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:1000
IP 0.2-1 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-TFPI Antibody, Rabbit Monoclonal 图片

Anti-TFPI rabbit monoclonal antibody at 1:500 dilution

Lane A: MCF7 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:35 kDa

Observed band size:40 kDa

TFPI was immunoprecipitated using:

Lane A:0.5 mg MCF-7 Whole Cell Lysate

0.5 µL anti-TFPI rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-TFPI rabbit monoclonal antibody,at 1:1000 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 35 kDa

Observed band size: 45 kDa

Recombinant Anti-TFPI Antibody, Rabbit Monoclonal: 别称

Anti-EPI Antibody; Anti-LACI Antibody; Anti-TFI Antibody; Anti-TFPI1 Antibody

TFPI 背景信息

Tissue factor pathway inhibitor (TFPI) is the natural inhibitor of TF coagulant and signaling activities. It is a Kunitz-type serine proteinase inhibitor that down-regulates tissue factor-initiated blood coagulation. With its Kunitz domains, TFPI exhibits significant homology with human inter-alpha-trypson inhibitor and bovin basic pancreatic trypsin inhibitor. TFPI is the natural inhibitor of TF coagulant and signaling activities. The importance of TFPI in the regulation of blood coagulation is emphasized by how its activity is modulated in human disease. In a factor (F) Xa-dependent feedback system, TFPI binds directly and inhibits the TF-FVII/FVIIa complex. Normally, TFPI exists in plasma both as a full-length molecule and as variably carboxy-terminal truncated forms. TFPI also circulates in complex with plasma lipoproteins. The levels and the dual inhibitor effect of TFPI on FXa and TF-FVII/FVIIa complex offers insight into the mechanisms of various pathological conditions triggered by TF. TFPI may play an important role in modulating TF-induced thrombogenesis and it may also provide a unique therapeutic approach for prophylaxis and/or treatment of various diseases. In addition, studies have shown that TFPI exhibits antiangiogenic and antimetastatic effects in vitro and in vivo. In animal models of experimental metastasis, both circulating and tumor cell-associated TFPI are shown to significantly reduce tumor cell-induced coagulation activation and lung metastasis.
全称
tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
参考文献
  • Lwaleed BA, et al. (2006) Tissue factor pathway inhibitor: structure, biology and involvement in disease. J Pathol. 208(3): 327-39.
  • Amirkhosravi A, et al. (2007) The role of tissue factor pathway inhibitor in tumor growth and metastasis. Semin Thromb Hemost. 33(7): 643-52.
  • Maroney SA, et al. (2008) Expression of tissue factor pathway inhibitor by endothelial cells and platelets. Transfus Apher Sci. 38(1): 9-14.
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