Anti-Jagged 1 Antibody, Rabbit Polyclonal

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Anti-Jagged 1 Antibody, Rabbit Polyclonal 产品信息

产品名称
Anti-Jagged 1 Antibody, Rabbit Polyclonal
经验证的应用
IHC-P,ICC/IF
交叉反应
Reacts with: Mouse
Predicted to React with: Human, Rat (Species predicted to react based on 100% sequence homology)
特异性
Mouse Jagged 1
免疫原
A synthetic peptide corresponding to the C-terminus of the Mouse Jagged 1/Jag1
制备方法
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Mouse Jagged 1/Jag1, and purified by antigen affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
PBS, pH7.0 with 0.03% Proclin300
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.

Anti-Jagged 1 Antibody, Rabbit Polyclonal 经验证的应用

应用 推荐稀释比/用量
IHC-P 1:100-1:400
ICC-IF 1:100-1:500
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Jagged 1 Antibody, Rabbit Polyclonal 图片

Immunochemical staining of mouse Jag1 in mouse kidney with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.
Immunofluorescence staining of Jag1 in RAW264.7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Mouse Jag1 polyclonal antibody (dilution ratio 1:200) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm and cell membrane.

Citation List

Anti-Jagged 1 Antibody, Rabbit Polyclonal: 别称

Anti-ABE2 Antibody; Anti-Gsfabe2 Antibody; Anti-Htu Antibody; Anti-Ozz Antibody; Anti-Ser-1 Antibody

Jagged 1 背景信息

Protein Jagged 1, also known as JAG1, JAGL1 and CD339, is a single-pass type I membrane protein which contains 1DSL domain and 15EGF-like domains. JAG1/Jagged 1 is widely expressed in adult and fetal tissues. The expression of JAG1/Jagged 1 is up-regulated in cervical squamous cell carcinoma. JAG1/Jagged 1 is also expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells. JAG1/Jagged 1 is a ligand for multiple Notch receptors. It is involved in the mediation of Notch signaling. JAG1/Jagged 1 may be involved in cell-fate decisions during hematopoiesis. JAG1/Jagged 1 seems to be involved in early and late stages of mammalian cardiovascular development. It inhibits myoblast differentiation and enhances fibroblast growth factor-induced angiogenesis. Defects in JAG1/Jagged 1 are the cause of Alagille syndrome type 1 (ALGS1). Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. Defects in JAG1/Jagged 1 are also a cause of tetralogy of Fallot (TOF). TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation.
全称
jagged 1
研究领域
参考文献
  • Oda T.et al., 1997, Nat. Genet. 16:235-242.
  • Krantz I.D. et al., 1998, Am. J. Hum. Genet. 62:1361-1369.
  • Li L. et al., 1998, Immunity. 8:43-55.
  • Jones E.A. et al., 2000, J. Med. Genet. 37: 658-662.
  • Roepke A.et al., 2003, Hum. Mutat. 21:100-100.
  • Jurkiewicz D.et al., 2005, Hum. Mutat. 25:321-321.
  • Warthen D.M.et al., 2006, Hum. Mutat. 27:436-443.
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