Anti-Leptin Receptor Antibody, Rabbit Polyclonal

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Anti-Leptin Receptor Antibody, Rabbit Polyclonal 产品信息

产品名称
Anti-Leptin Receptor Antibody, Rabbit Polyclonal
经验证的应用
WB,IHC-P
交叉反应
Reacts with: Mouse
特异性
Mouse Leptin Receptor
免疫原
A synthetic peptide corresponding to the center region of the Mouse Leptin Receptor/LEPR
制备方法
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse Leptin Receptor/LEPR, and purified by antigen affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
PBS, pH7.0 with 0.03% Proclin300
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.

Anti-Leptin Receptor Antibody, Rabbit Polyclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
IHC-P 1:200-1:1000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Leptin Receptor Antibody, Rabbit Polyclonal 图片

Anti-LEPR rabbit polyclonal antibody at 1:500 dilution

Lane A: Mouse brain tissue lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:132 kDa

Observed band size:125 kDa

Immunochemical staining of mouse LEPR in mouse liver with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.

Anti-Leptin Receptor Antibody, Rabbit Polyclonal: 别称

Anti-db Antibody; Anti-diabetes Antibody; Anti-Leprb Antibody; Anti-LEPROT Antibody; Anti-Modb1 Antibody; Anti-OB-RGRP Antibody; Anti-obese-like Antibody; Anti-obl Antibody; Anti-Obr Antibody

Leptin Receptor 背景信息

Leptin Receptor or CD295 belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight) and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Leptin Receptor/CD295 is transmembrane catalytic receptors found on NPY/AgRP and alpha-MSH/CART neurons in hypothalamic nuclei. Leptin receptors (Ob-Rs) are coded for by one human gene that produces six different isoforms; Ob-Ra - Ob-Rf. Ob-Rs exist as constitutive dimers at physiological expression levels. Only the Ob-Rb isoform can transduce intracellular signals and does so through activation of the JAK2/STAT3, PI 3-K, and MAPK signaling cascades. Activation of Ob-Rs mediates transcriptional regulation of the hypothalamic melanocortin pathway and downregulates endocannabinoid expression. Leptin acts via leptin receptors. Leptin resistance has been proposed as a pathophysiological mechanism of obesity. In obese individuals, Ob-Ra (which is involved in the active transport of leptin across the blood-brain barrier) expression is downregulated and the individual may be unresponsive to leptin signals. Ob-R antagonists are of great interest in the development of pharmacological treatments for obesity. Mutations in the Leptin Receptor/CD295 have been associated with obesity and pituitary dysfunction.
全称
leptin receptor
参考文献
  • Heshka JT, et al. (2001) A role for dietary fat in leptin receptor, OB-Rb, function. Life Sci. 69 (9): 987-1003.
  • Chen H, et al (1996) Evidence that the diabetes gene encodes the leptin receptor: identification of a mutation in the leptin receptor gene in db/db mice. Cell. 84 (3): 491-5.
  • Bjrbaek C, et al. (1998) Divergent signaling capacities of the long and short isoforms of the leptin receptor. J Biol Chem. 272 (51): 32686-95.
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