Anti-M13 Antibody (HRP), Mouse Monoclonal

RRID Number: AB_2857928
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Anti-M13 Antibody (HRP), Mouse Monoclonal 产品信息

产品名称
Anti-M13 Antibody (HRP), Mouse Monoclonal
经验证的应用
ELISA
交叉反应
Reacts with: other
特异性
other M13
免疫原
M13 Bacteriophage
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with M13 phage. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography and then conjugated with Horseradish-peroxidase (HRP).
来源
Monoclonal Mouse IgG1 Clone #MM05
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
HRP
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Protected from prolonged exposure to light. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-M13 Antibody (HRP), Mouse Monoclonal 经验证的应用

应用 推荐稀释比/用量
ELISA 0.1-0.4 μg/mL
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-M13 Antibody (HRP), Mouse Monoclonal 图片

Coating: Coat corresponding Protein (20 μg/mL in pH 9.6 carbonate buffer) in NUNC plate
Sample: Add the indicated amounts of M13 Bacteriophages displaying Rabbit/Mouse Fab targeted for different proteins;
Primary Antibody: M13 Antibody (11973-MM05T), 0.365 μg/mL
Secondary Antibody: anti-mouse F(ab)2 Antibody/HRP

M13 背景信息

M13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 647 nucleotides long encapsulated in approximately 27 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. Infection with filamentous phages is not lethal, however the infection causes turbid plaques in E. coli. It is a non-lytic virus. However a decrease in the rate of cell growth is seen in the infected cells. M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures and nanotechnology. The phage coat is primarily assembled from a 5 amino acid protein called pVIII (or p8), which is encoded by gene VIII (or g8) in the phage genome. For a wild type M13 particle, it takes about approximately 27 copies of p8 to make the coat about 9 nm long. The coat's dimensions are flexible though and the number of p8 copies adjusts to accommodate the size of the single stranded genome it packages. The general stages to a viral life cycle are: infection, replication of the viral genome, assembly of new viral particles and then release of the progeny particles from the host. Filamentous phage use a bacterial structure known as the F pilus to infect E. coli, with the M13 p3 tip contacting the TolA protein on the bacterial pilus. The phage genome is then transferred to the cytoplasm of the bacterial cell where resident proteins convert the single stranded DNA genome to a double stranded replicative form.
参考文献
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  • Identification of a peptide for folate receptor alpha by phage display and its tumor targeting activity in ovary cancer xenograft
    Author
    Xing, L;Xu, Y;Sun, K;Wang, H;Zhang, F;Zhou, Z;Zhang, J;Zhang, F;Caliskan, B;Qiu, Z;Wang, M;
    Year
    2018
    Journal
    Sci Rep
    Application
    ELISA (phage display)
  • Sensitive detection of dengue virus NS1 by highly stable affibody-functionalized gold nanoparticles
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    Bang, J;Park, H;Choi, W;Sung, D;Lee, J;Lee, K;Kim, S;
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    Year
    2019
    Journal
    Antibodies
    Application
    biopanning
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    Author
    Dong, S;Gao, M;Bo, Z;Guan, L;Hu, X;Zhang, H;Liu, B;Li, P;He, K;Liu, X;Zhang, C;
    Year
    2020
    Journal
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    Application
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  • Blocking antibodies against SARS-CoV-2 RBD isolated from a phage display antibody library using a competitive biopanning strategy
    Author
    Zeng, X;Li, L;Lin, J;Li, X;Liu, B;Kong, Y;Zeng, S;Du, J;
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    Journal
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    Author
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    Author
    Cho, SH;Kil, EJ;Cho, S;Byun, HS;Kang, EH;Choi, HS;Lee, MG;Lee, JS;Lee, YG;Lee, S;
    Year
    2020
    Journal
    Sci Rep
    Application
    phag Elisa
  • Abstracts from the 26th European Society for Animal Cell Technology Meeting - Cell culture technologies: bridging academia and industry to provide solutions for patients
    Author
    Pybus, LP;Hawke, E;Knowles, C;Kalsi, D;Barber, N;
    Year
    2020
    Journal
    BMC Proc
    Application
    cell panning
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