Anti-alanyl-tRNA synthetase Antibody, Rabbit Polyclonal

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Anti-alanyl-tRNA synthetase Antibody, Rabbit Polyclonal 产品信息

产品名称
Anti-alanyl-tRNA synthetase Antibody, Rabbit Polyclonal
经验证的应用
WB,ELISA,IHC-P,ICC/IF,IP
交叉反应
Reacts with: Mouse
特异性
Mouse alanyl-tRNA synthetase
免疫原
Recombinant Mouse AARS / alanyl-tRNA synthetase Protein (Catalog#50384-M08B)
制备方法
Produced in rabbits immunized with purified, recombinant Mouse AARS / alanyl-tRNA synthetase (rM AARS / alanyl-tRNA synthetase; Catalog#50384-M08B; Q8BGQ7; Met1-Asn968). AARS / alanyl-tRNA synthetase specific IgG was purified by Mouse AARS / alanyl-tRNA synthetase affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-alanyl-tRNA synthetase Antibody, Rabbit Polyclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:1000
ELISA 1:25000-1:50000
IHC-P 1:2500-1:15000
ICC-IF 1:1000-1:5000
IP 0.2-1 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-alanyl-tRNA synthetase Antibody, Rabbit Polyclonal 图片

Immunofluorescence staining of AARS in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-mouse AARS polyclonal antibody (1:5000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Immunochemical staining of mouse AARS in mouse stomach with rabbit polyclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of mouse AARS in mouse kidney with rabbit polyclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections.

Mouse AARS was immunoprecipitated using:

Lane A:0.5 mg K562 Whole Cell Lysate

0.5 µL anti-Mouse AARS rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-Mouse AARS rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 107 kDa

Observed band size: 107 kDa

Anti-AARS rabbit polyclonal antibody at 1:500 dilution

Lane A: NIH3T3 Whole Cell Lysate

Lane B: Jurkat Whole Cell Lysate

Lane C: K562 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:107 kDa

Observed band size:107 kDa

(We are unsure as to the identity of these extra bands.)

Anti-alanyl-tRNA synthetase Antibody, Rabbit Polyclonal: 别称

Anti-AI316495 Antibody; Anti-C76919 Antibody; Anti-sti Antibody

alanyl-tRNA synthetase 背景信息

Alanyl-tRNA synthetase (AARS) belongs to the family of ligases, specifically those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. This enzyme participates in alanine and aspartate metabolism and aminoacyl-tRNA biosynthesis. Alanyl-tRNA synthetase (AlaRS) catalyzes synthesis of Ala-tRNA (Ala) and hydrolysis of mis-acylated Ser- and Gly-tRNA (Ala) at 2 different catalytic sites. Their role is not confined to catalyze the attachment of amino acids to transfer RNAs and thereby establish the rules of genetic code by virtue of matching the nucleotide triplet of anticodon with cognate amino acid. Under apoptotic conditions in cell culture, the full-length enzyme is secreted, and the two cytokine activities can be generated by leukocyte elastase, an extracellular protease. Secretion of this tRNA synthetase may contribute to apoptosis both by arresting translation and producing needed cytokines. This protein could be an attractive target of drugs against bacterial, fungal and parasitic infections.
全称
alanyl-tRNA synthetase
参考文献
  • Wakasugi K, et al. (1999) Two Distinct Cytokines Released from a Human Aminoacyl-tRNA Synthetase. Science. 284 (5411): 147-51.
  • Sokabe M, et al. (2009) The structure of alanyl-tRNA synthetase with editing domain. Proc Natl Acad Sci . 106 (27): 11028-33.
  • Skupinska M, et al. (2009) AARS--the etiological factor and the attractive target of many disorders. Postepy Biochem. 55 (4): 373-84.
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