Recombinant Anti-Carbonic Anhydrase 8/CA8 Antibody, Rabbit Monoclonal

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Recombinant Anti-Carbonic Anhydrase 8/CA8 Antibody, Rabbit Monoclonal 产品信息

产品名称
Recombinant Anti-Carbonic Anhydrase 8/CA8 Antibody, Rabbit Monoclonal
经验证的应用
WB,IP
交叉反应
Reacts with: Mouse
特异性
Mouse Carbonic Anhydrase 8/CA8
免疫原
Recombinant Mouse Carbonic Anhydrase VIII / CA8 protein (Catalog#50767-M08E)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Carbonic Anhydrase VIII / CA8 (rM Carbonic Anhydrase VIII / CA8; Catalog#50767-M08E; P28651; Met1-Gln291).
来源
Monoclonal Rabbit IgG Clone #005
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-Carbonic Anhydrase 8/CA8 Antibody, Rabbit Monoclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-Carbonic Anhydrase 8/CA8 Antibody, Rabbit Monoclonal 图片

Anti-CA8 rabbit monoclonal antibody at 1:500 dilution

Lane A: 293T Whole Cell Lysate

Lane B: A549 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:33 kDa

Observed band size:36 kDa

Mouse CA8 was immunoprecipitated using:

Lane A:0.5 mg 293T Whole Cell Lysate

Lane B:0.5 mg A549 Whole Cell Lysate

2 µL anti-Mouse CA8 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Mouse CA8 rabbit monoclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 33 kDa

Observed band size: 37 kDa

Recombinant Anti-Carbonic Anhydrase 8/CA8 Antibody, Rabbit Monoclonal: 别称

Anti-AW546993 Antibody; Anti-Ca8 Antibody; Anti-Cals Antibody; Anti-Cals1 Antibody; Anti-Car8 Antibody; Anti-Carp Antibody; Anti-RP23-180H12.1 Antibody; Anti-wdl Antibody

Carbonic Anhydrase 8/CA8 背景信息

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer.
全称
carbonic anhydrase VIII
参考文献
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
  • Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.
  • Miyaji E, et al. (2003) Overexpression of carbonic anhydrase-related protein VIII in human colorectal cancer. The Journal of Pathology. 201(1): 37-45.
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