Recombinant Mouse LY9 / CD229 / SLAMF3 protein (Catalog#50504-M08H)
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse LY9 / CD229 / SLAMF3 (rM LY9 / CD229 / SLAMF3; Catalog#50504-M08H; NP_032560.2; Met1-Arg453).
Monoclonal Rabbit IgG Clone #002
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Flow cytometric analysis of Mouse Ly9(CD229) expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse Ly9(CD229), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence staining of mouse Ly9 in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse Ly9 monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).