Recombinant Anti-EpCAM Antibody, Rabbit Monoclonal

RRID Number: AB_2860513
EliteRmab®, manufactured by recombinant technology
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Recombinant Anti-EpCAM Antibody, Rabbit Monoclonal 产品信息

产品名称
Recombinant Anti-EpCAM Antibody, Rabbit Monoclonal
经验证的应用
WB,ELISA,ELISA(Det),IHC-P
交叉反应
Reacts with: Mouse
特异性
Mouse EpCAM
No cross-reactivity in ELISA with
Human EPCAM
免疫原
Recombinant Mouse EpCAM / TROP-1 / TACSTD1 protein (Catalog#50591-M08H)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse EpCAM / TROP-1 / TACSTD1 (rM EpCAM / TROP-1 / TACSTD1; Catalog#50591-M08H; NP_032558.2; Met 1-Thr 266).
来源
Monoclonal Rabbit IgG Clone #002
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-EpCAM Antibody, Rabbit Monoclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:1000
ELISA 1:25000-1:50000
IHC-P 1:500-1:2000
ELISA(Det) 1:5000-1:50000
Notes
ELISA(Det): This antibody will detect Mouse EpCAM / TROP-1 / TACSTD1 in ELISA pair set (Catalog: # SEK50591). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 50591-R052).
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-EpCAM Antibody, Rabbit Monoclonal 图片

Immunochemical staining of mouse EpCAM in mouse uterus with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of mouse EpCAM in mouse stomach with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of mouse EpCAM in mouse intestine with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.

Anti-EPCAM rabbit monoclonal antibody at 1:500 dilution

Lane A: 293T Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:35 kDa

Observed band size:38 kDa

Recombinant Anti-EpCAM Antibody, Rabbit Monoclonal: 别称

Anti-CD326 Antibody; Anti-EGP Antibody; Anti-EGP-2 Antibody; Anti-Egp314 Antibody; Anti-Ep-CAM Antibody; Anti-EpCAM1 Antibody; Anti-GA733-2 Antibody; Anti-gp40 Antibody; Anti-Ly74 Antibody; Anti-Tacsd1 Antibody; Anti-Tacstd1 Antibody; Anti-TROP1 Antibody

EpCAM 背景信息

Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type â…transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.
全称
epithelial cell adhesion molecule
参考文献
  • Brunner A, et al. (2008) EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder. J Clin Pathol. 61(3):307-10.
  • Trzpis M, et al. (2008) EpCAM in morphogenesis. Front Biosci. 13: 5050-5.
  • Munz M, et al. (2009) The emerging role of EpCAM in cancer and stem cell signaling. Cancer Res. 69(14): 5627-9.
  • Carpenter G, et al. (2009) EpCAM: another surface-to-nucleus missile. Cancer Cell. 15(3): 165-6.
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