Recombinant Anti-Fas/CD95 Antibody, Rabbit Monoclonal

EliteRmab®, manufactured by recombinant technology
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Recombinant Anti-Fas/CD95 Antibody, Rabbit Monoclonal 产品信息

产品名称
Recombinant Anti-Fas/CD95 Antibody, Rabbit Monoclonal
经验证的应用
FCM,ICC/IF
交叉反应
Reacts with: Mouse
特异性
Mouse Fas/CD95
免疫原
Recombinant Mouse CD95 / APO-1 / TNFRSF6 / FAS Protein (Catalog#50027-M08H)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD95 / APO-1 / TNFRSF6 / FAS (rM CD95 / APO-1 / TNFRSF6 / FAS; Catalog#50027-M08H; NP_032013.2; Met1-Arg169).
来源
Monoclonal Rabbit IgG Clone #004
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-Fas/CD95 Antibody, Rabbit Monoclonal 经验证的应用

应用 推荐稀释比/用量
ICC-IF 1:20-1:100
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-Fas/CD95 Antibody, Rabbit Monoclonal 图片

Immunofluorescence staining of mouse FAS in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse FAS monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody (red).
Flow cytometric analysis of Mouse FAS(CD95) expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse FAS(CD95), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Recombinant Anti-Fas/CD95 Antibody, Rabbit Monoclonal: 别称

Anti-AI196731 Antibody; Anti-APO1 Antibody; Anti-APT1 Antibody; Anti-CD95 Antibody; Anti-lpr Antibody; Anti-TNFR6 Antibody; Anti-Tnfrsf6 Antibody

Fas/CD95 背景信息

CD95 (APO-1/Fas) is an important inducer of the extrinsic apoptosis signaling pathway and therapy induced apoptosis of many tumor cells has been linked to the activity of CD95. is a prototype death receptor characterized by the presence of an 80 amino acid death domain in its cytoplasmic tail. This domain is essential for the recruitment of a number of signaling components upon activation by either agonistic anti-CD95 antibodies or cognate CD95 ligand that initiate apoptosis. The complex of proteins that forms upon triggering of CD95 is called the death-inducting signaling complex (DISC). The DISC consists of an adaptor protein and initiator caspases and is essential for induction of apoptosis. CD95 is also crucial for the negative selection of B cells within the germinal center (GC). Impairment of CD95-mediated apoptosis results in defective affinity maturation and the persistence of autoreactive B-cell clones. Changes in the expression of CD95 and/or its ligand CD95L are frequently found in human cancer. The downregulation or mutation of CD95 has been proposed as a mechanism by which cancer cells avoid destruction by the immune system through reduced apoptosis sensitivity. Thus, CD95 has therefore been viewed as a tumor suppressor. CD95 has been reported to be involved in the activation of NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and the alternate pathways for CTL-mediated cytotoxicity. Accordingly, this protein is implicated in the pathogenesis of various malignancies and diseases of the immune system. The CD95/CD95L system was implicated in the etiology of inflammatory bowel disease (IBD) based, primarily, on the finding that CD95 is highly expressed in the intestinal epithelial cells and that epithelial apoptosis is increased in IBD.
全称
Fas cell surface death receptor
研究领域
Related Pathways
  • Death Receptor Signaling
    Death Receptor Signaling
  • p53 Pathway
    p53 Pathway
  • AMPK Signaling Pathway
    AMPK Signaling Pathway
参考文献
  • Mschen M, et al. (2002) The origin of CD95-gene mutations in B-cell lymphoma. Trends Immunol. 23(2): 75-80.
  • Peter ME, et al. (2003) The CD95(APO-1/Fas) DISC and beyond. Cell Death Differ. 10(1): 26-35.
  • Peter ME, et al. (2005) Does CD95 have tumor promoting activities Biochim Biophys Acta. 1755(1): 25-36.
  • Chen L, et al. (2010) Cell death in the colonic epithelium during inflammatory bowel diseases: CD95/Fas and beyond. Inflamm Bowel Dis. 16(6): 1071-6.
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