Recombinant Anti-FCGR4 Antibody, Rabbit Monoclonal

EliteRmab®, manufactured by recombinant technology
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Recombinant Anti-FCGR4 Antibody, Rabbit Monoclonal 产品信息

产品名称
Recombinant Anti-FCGR4 Antibody, Rabbit Monoclonal
经验证的应用
ELISA,FCM,ICC/IF
交叉反应
Reacts with: Mouse
特异性
Mouse FCGR4
Has cross-reactivity in ELISA with
Human CD64 / FCGR1A
No cross-reactivity in ELISA with
Human CD16a / FCGR3A / FcRIIIa
Human CD16b / FCGR3B / FcRIIIb
Human CD32a / FCGR2A
Human CD32b / FCGR2B
Mouse CD16 / FCGR3
免疫原
Recombinant Mouse FCGR4 protein (Catalog#50036-M08H)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse FCGR4 (rM FCGR4; Catalog#50036-M08H; NP_653142.2; Met 1-Gln 203).
来源
Monoclonal Rabbit IgG Clone #012
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-FCGR4 Antibody, Rabbit Monoclonal 经验证的应用

应用 推荐稀释比/用量
ELISA 1:5000-1:10000
ICC-IF 1:50-1:1000
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-FCGR4 Antibody, Rabbit Monoclonal 图片

Immunofluorescence staining of Mouse FcGR4 in RAW264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Mouse FcGR4 monoclonal antibody (1:100) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.
Flow cytometric analysis of FCGR4 expression on Raw264.7 cells. Mouse leukaemic monocyte macrophage cell line RAW 264.7 were stained with anti-mouse FCGR4 (50036-R012), then a FITC-conjugated second step antibody. The histogram were derived from the gated events based on light scattering characteristics of lymphocytes.

Recombinant Anti-FCGR4 Antibody, Rabbit Monoclonal: 别称

Anti-4833442P21Rik Antibody; Anti-CD16-2 Antibody; Anti-FcgammaRIV Antibody; Anti-Fcgr3a Antibody; Anti-FcgRIV Antibody; Anti-Fcrl3 Antibody

FCGR4 背景信息

全称
Fc receptor, IgG, low affinity IV
研究领域
  • A noncanonical role for the engulfment gene ELMO1 in neutrophils that promotes inflammatory arthritis
    Author
    Arandjelovic, S;Perry, JSA;Lucas, CD;Penberthy, KK;Kim, TH;Zhou, M;Rosen, DA;Chuang, TY;Bettina, AM;Shankman, LS;Cohen, AH;Gaultier, A;Conrads, TP;Kim, M;Elliott, MR;Ravichandran, KS;
    Year
    2019
    Journal
    Nat. Immunol.
    Application
    FCM
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