Mouse FCGR4 Has cross-reactivity in ELISA with Human CD64 / FCGR1A No cross-reactivity in ELISA with Human CD16a / FCGR3A / FcRIIIa
Human CD16b / FCGR3B / FcRIIIb
Human CD32a / FCGR2A
Human CD32b / FCGR2B
Mouse CD16 / FCGR3
Recombinant Mouse FCGR4 protein (Catalog#50036-M08H)
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse FCGR4 (rM FCGR4; Catalog#50036-M08H; NP_653142.2; Met 1-Gln 203).
Monoclonal Rabbit IgG Clone #012
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunofluorescence staining of Mouse FcGR4 in RAW264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Mouse FcGR4 monoclonal antibody (1:100) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.
Flow cytometric analysis of FCGR4 expression on Raw264.7 cells. Mouse leukaemic monocyte macrophage cell line RAW 264.7 were stained with anti-mouse FCGR4 (50036-R012), then a FITC-conjugated second step antibody. The histogram were derived from the gated events based on light scattering characteristics of lymphocytes.