Recombinant Anti-FGFRL1 Antibody, Rabbit Monoclonal

EliteRmab®, manufactured by recombinant technology
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Recombinant Anti-FGFRL1 Antibody, Rabbit Monoclonal 产品信息

产品名称
Recombinant Anti-FGFRL1 Antibody, Rabbit Monoclonal
经验证的应用
WB,IP
交叉反应
Reacts with: Mouse
特异性
Mouse FGFRL1
免疫原
Recombinant Mouse FGFRL1 / FGFR5 protein (Catalog#50182-M08H)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse FGFRL1 / FGFR5 (rM FGFRL1 / FGFR5; Catalog#50182-M08H; NP_473412.1; Met1-Pro374).
来源
Monoclonal Rabbit IgG Clone #019
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-FGFRL1 Antibody, Rabbit Monoclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
IP 4-6 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-FGFRL1 Antibody, Rabbit Monoclonal 图片

Anti-FGFRL1 rabbit monoclonal antibody at 1:500 dilution

Lane A: 293T Whole Cell Lysate

Lane B: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:55 kDa

Observed band size:62 kDa

Mouse FGFRL1 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

2 µL anti-Mouse FGFRL1 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Mouse FGFRL1 rabbit monoclonal antibody,at 1:100 dilution

Secondary antibody:

Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution

Developed using the DAB staining technique.

Performed under reducing conditions.

Predicted band size: 55 kDa

Observed band size: 70 kDa

Recombinant Anti-FGFRL1 Antibody, Rabbit Monoclonal: 别称

Anti-FGFR5 Antibody; Anti-FGFR5beta Antibody; Anti-FGFR5gamma Antibody

FGFRL1 背景信息

Fibroblast growth factor receptor-like 1 (FGFRL1) also known as Fibroblast growth factor receptor 5 (FGFR5), is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. A unique feature of FGFRL1/FGFR5 is that it does not contain an intracellular tyrosine kinase domain. Some muscle types, including the muscles of the tongue and the diaphragm, express FGFRL1/FGFR5 at relatively high level. In contrast, the heart and the skeletal muscles of the limbs, as well as many other organs (brain, lung, liver, kidney, gut) express Fgfrl1 only at basal level. It is conceivable that FGFRL1/FGFR5 interacts with other Fgfrs, which are expressed in cartilage and muscle, to modulate FGF signaling.
全称
fibroblast growth factor receptor-like 1
参考文献
  • Wiedemann M, et al. (2000) Characterization of a novel protein (FGFRL1) from human cartilage related to FGF receptors. Genomics. 69(2): 275-9.
  • Trueb B, et al. (2006) Expression of FGFRL1, a novel fibroblast growth factor receptor, during embryonic development. Int J Mol Med. 17(4): 617-20.
  • Wiedemann M, et al. (2001) The mouse Fgfrl1 gene coding for a novel FGF receptor-like protein. Biochim Biophys Acta. 1520(3): 247-50.
  • Trueb B, et al. (2003) Characterization of FGFRL1, a novel fibroblast growth factor (FGF) receptor preferentially expressed in skeletal tissues. J Biol Chem. 278(36): 33857-65.
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