Anti-IL-9 Antibody, Rabbit Polyclonal

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Anti-IL-9 Antibody, Rabbit Polyclonal 产品信息

产品名称
Anti-IL-9 Antibody, Rabbit Polyclonal
经验证的应用
WB,IHC-P
交叉反应
Reacts with: Mouse
特异性
Mouse IL-9
免疫原
A synthetic peptide corresponding to the N-terminus of the mouse IL9
制备方法
Produced in rabbits immunized with A synthetic peptide corresponding to the N-terminus of the mouse IL9 , and purified by antigen affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-IL-9 Antibody, Rabbit Polyclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:1000
IHC-P 1:5000-1:25000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-IL-9 Antibody, Rabbit Polyclonal 图片

Immunochemical staining of mouse IL9 in mouse spleen (from 2 donors) with rabbit polyclonal antibody at 1:25000 dilution, formalin-fixed paraffin embedded sections.

Anti-IL9 rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:15 kDa

Observed band size:17 kDa

(We are unsure as to the identity of these extra bands.)

Anti-IL-9 Antibody, Rabbit Polyclonal: 别称

Anti-Il-9 Antibody; Anti-P40 Antibody

IL-9 背景信息

Interleukin 9, also known as IL-9, is a cytokine (cell signaling molecule) belonging to the group of interleukins. IL-9 is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL-9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness. IL-9 is a key molecule that affects the differentiation of TH17 cells and Treg function. IL-9 predominantly produced by TH17 cells synergizes with TGF-β1 to differentiate naive CD4+ T cells into TH17 cells, while IL-9 secretion by TH17 cells is regulated by IL-23. Interestingly, IL-9 enhances the suppressive functions of FoxP3+ CD4+ Treg cells in vitro, and the absence of IL-9 signaling weakens the suppressive activity of nTregs in vivo, leading to an increase in effector cells and worsening of experimental autoimmune encephalomyelitis. The mechanism of IL-9 effects on TH17 and Tregs is through activation of STAT3 and STAT5 signaling. Our findings highlight the role of IL-9 as a regulator of pathogenic versus protective mechanisms of immune responses.
全称
interleukin 9
Related Pathways
  • Common G-Chain signaling pathway
    Common G-Chain signaling pathway
参考文献
  • Elyaman W, et al. (2009) IL-9 induces differentiation of TH17 cells and enhances function of FoxP3+ natural regulatory T cells. Proc Natl Acad Sci U S A. 106(31): 12885-90.
  • Dong Q, et al. (1999) IL-9 induces chemokine expression in lung epithelial cells and baseline airway eosinophilia in transgenic mice. Eur J Immunol. 29(7): 2130-9.
  • Kimura Y, et al. (1995) Sharing of the IL-2 receptor gamma chain with the functional IL-9 receptor complex. Int Immunol. 7(1): 115-20.
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